The Potentiation Activity of Azithromycin in Combination with Colistin or Levofloxacin Pseudomonas Biofilm Infection

被引:1
作者
Wang, Yuhang [1 ]
Li, Chunsun [2 ]
Zhang, Huan [1 ]
Chi, Yulong [1 ]
Cai, Yun [1 ,3 ]
机构
[1] Peoples Liberat Army Gen Hosp, Ctr Med Clin Res, Dept Pharm, Med Supplies Ctr, Beijing, Peoples R China
[2] Peoples Liberat Army Gen Hosp, Lab Dept Pulm & Crit Care Med, Beijing, Peoples R China
[3] Peoples Liberat Army Gen Hosp, Ctr Med Clin Res, Dept Pharm, Med Supplies Ctr, 28 Fu Xing Rd, Beijing 100853, Peoples R China
关键词
Pseudomonas aeruginosa; biofilm; azithromycin; combinations; AERUGINOSA BIOFILM; ANTIBIOTICS; AMIKACIN;
D O I
10.2147/IDR.S438576
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objective: Pseudomonas aeruginosa (PA) often displays drug resistance and biofilm-mediated adaptability. Here, we aimed to evaluate the antibiofilm efficacy of azithromycin-based combination regimens. Methods: Minimum inhibitory concentrations (MICs), minimal biofilm eradication concentrations (MBECs), and MBEC-combination of azithromycin, colistin, amikacin, and levofloxacin to bioluminescent strain PAO1 and carbapenem-resistant PAO1 (CRPAO1) were assessed. An animal biofilm infection model was established and detected using a live animal bio-photonic imaging system. Results: In vitro, PAO1 and CRPAO1 were susceptible to colistin, amikacin, and levofloxacin, while they were unsusceptible to azithromycin. The combinations based on azithromycin have no synergistic effect on biofilm in vitro. In vivo, azithromycin plus colistin or levofloxacin could shorten the PAO1 biofilm eradication time, which totally eradicates the biofilm in all mice on the 8th or 6th day, while monotherapy only eradicate biofilm in 70% or 80% mice on the 8th day. For CRPAO1 biofilm, only azithromycin- colistin combination and colistin monotherapy eradicated the bacteria in 60% and 40% of mice at the 6th day. Conclusion: Azithromycin-based combinations containing levofloxacin or colistin had no synergistic effect in vitro, and they are promising for clinical applications due to the good synergistic activity against PAO1 biofilms in vivo.
引用
收藏
页码:1259 / 1266
页数:8
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