Clinical evaluation of commercial SARS-CoV-2 serological assays in a malaria endemic setting

被引：3

作者：

Dabitao, Djeneba ^{[1
,2
,6
,7
]}

Shaw-Saliba, Kathryn ^{[3
]}

Konate, Drissa S. ^{[1
,2
]}

Highbarger, Helene C. ^{[4
]}

Lallemand, Perrine ^{[4
]}

Sanogo, Ibrahim ^{[1
,2
]}

Rehman, Tauseef ^{[4
]}

Wague, Mamadou ^{[1
,2
]}

Coulibaly, Nadie ^{[1
,2
]}

Kone, Bourahima ^{[1
,2
]}

Baya, Bocar ^{[1
,2
]}

Diakite, Seidina A. S. ^{[1
,2
]}

Samake, Seydou ^{[1
,2
]}

Akpa, Esther ^{[3
]}

Tounkara, Moctar ^{[1
,2
]}

Laverdure, Sylvain ^{[5
]}

Doumbia, Seydou ^{[1
,2
]}

Lane, H. Clifford ^{[3
]}

Diakite, Mahamadou ^{[1
,2
]}

Dewar, Robin L. ^{[4
]}

机构：

[1] Univ Sci Tech & Technol Bamako USTTB, Univ Clin Res Ctr UCRC, Fac Pharm, Bamako, Mali

[2] Univ Sci Tech & Technol Bamako USTTB, Fac Med & Odonto Stomatol, Bamako, Mali

[3] NIAID, Collaborat Clin Res Branch CCRB, Div Clin Res DCR, Bethesda, MD USA

[4] Virus Isolat & Serol Lab, Frederick Natl Lab FNL, Frederick, MD USA

[5] Frederick Natl Lab FNL, Lab Human Retrovirol & Immunoinformat, Frederick, MD USA

[6] Univ Sci Tech & Technol Bamako USTTB, Univ Clin Res Ctr UCRC, Fac Pharm, BP 1805, Bamako, Mali

[7] Univ Sci Tech & Technol Bamako USTTB, Fac Med & Odonto Stomatol, BP 1805, Bamako, Mali

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2023年 / 517卷

基金：

美国国家卫生研究院;

关键词：

COVID-19; Antibodies; Validation; Plasmodium falciparum; Mali; REACTIVITY; COVID-19;

D O I：

10.1016/j.jim.2023.113488

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The levels of immune response to SARS-CoV-2 infection or vaccination are poorly understood in African populations and is complicated by cross-reactivity to endemic pathogens as well as differences in host responsiveness. To begin to determine the best approach to minimize false positive antibody levels to SARS-CoV-2 in an African population, we evaluated three commercial assays, namely Bio-Rad Platelia SARS-CoV-2 Total Antibody (Platelia), Quanterix Simoa Semi-Quantitative SARS-CoV-2 IgG Antibody Test (anti-Spike), and the GenScript cPassTM SARS-CoV-2 Neutralization Antibody Detection Kit (cPass) using samples collected in Mali in West Africa prior to the emergence of SARS-CoV-2. A total of one hundred samples were assayed. The samples were categorized in two groups based on the presence or absence of clinical malaria. Overall, thirteen out of one hundred (13/100) samples were false positives with the Bio-Rad Platelia assay and one of the same one hundred (1/100) was a false positive with the anti-Spike IgG Quanterix assay. None of the samples tested with the GenScript cPass assay were positive. False positives were more common in the clinical malaria group, 10/50 (20%) vs. the nonmalaria group 3/50 (6%); p = 0.0374 using the Bio-Rad Platelia assay. Association between false positive results and parasitemia by Bio-Rad remained evident, after adjusting for age and sex in multivariate analyses. In summary, the impact of clinical malaria on assay performance appears to depend on the assay and/or antigen being used. A careful evaluation of any given assay in the local context is a prerequisite for reliable serological assessment of anti-SARS-CoV-2 humoral immunity.

引用

页数：7

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