Primary infection of BALB/c mice with a dengue virus type 4 strain leads to kidney injury

被引：2

作者：

Rasinhas, Arthur da Costa ^{[1
,2
]}

Jacome, Fernanda Cunha ^{[1
,2
]}

Caldas, Gabriela Cardoso ^{[1
,3
]}

Teixeira de Almeida, Ana Luisa ^{[1
]}

Coutinho de Souza, Daniel Dias ^{[1
]}

Rodrigues dos Santos, Joao Paulo ^{[3
]}

Dias, Helver Goncalves ^{[2
]}

Araujo, Eduarda Lima ^{[1
]}

Mohana-Borges, Ronaldo ^{[4
]}

Barth, Ortrud Monika ^{[1
]}

dos Santos, Flavia Barreto ^{[2
]}

Barreto-Vieira, Debora Ferreira ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Lab Morfol & Morfogenese Viral, Rio De Janeiro, RJ, Brazil

[2] Fundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Lab Imunol Viral, Rio De Janeiro, RJ, Brazil

[3] Fundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Lab Patol, Rio De Janeiro, RJ, Brazil

[4] Univ Fed Rio De Janeiro, Inst Biofis Carlos Chagas Filho, Lab Biotecnol & Bioengn Estrutural, Rio De Janeiro, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2023年 / 118卷

关键词：

DENV-4-BALB; c mice; kidney; histopathology; ultrastructure; FEVER; RHABDOMYOLYSIS; MANIFESTATIONS; PATHOGENESIS; ANTIBODY; CELLS;

D O I：

10.1590/0074-02760220255

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

BACKGROUND Dengue is a disease caused by dengue virus (DENV-1 through-4). Among the four serotypes, DENV-4 remains the least studied. Acute kidney injury is a potential complication of dengue generally associated with severe dengue infection. OBJECTIVES The goal of this study was to investigate the alterations caused by experimental dengue infection in the kidney of adult BALB/c mice. METHODS In this study, BALB/c mice were infected through the intravenous route with a DENV-4 strain, isolated from a human patient. The kidneys of the mice were procured and subject to histopathological and ultrastructural analysis. FINDINGS The presence of the viral antigen was confirmed through immunohistochemistry. Analysis of tissue sections revealed the presence of inflammatory cell infiltrate throughout the parenchyma. Glomerular enlargement was a common find. Necrosis of tubular cells and haemorrhage were also observed. Analysis of the kidney on a transmission electron microscope allowed a closer look into the necrotic tubular cells, which presented nuclei with condensed chromatin, and loss of cytoplasm. MAIN CONCLUSIONS Even though the kidney is probably not a primary target of dengue infection in mice, the inoculation of the virus in the blood appears to damage the renal tissue through local inflammation.

引用

页数：10