Rapid detection of varicella-zoster virus based on an immunochromatographic strip

被引：3
|
作者：
Wang, Aiping [1 ,2 ]
Niu, Yan [2 ]
Zhao, Jianguo [1 ,3 ]
Liu, Hongliang [1 ,2 ]
Ding, Peiyang [1 ,2 ]
Chen, Yumei [2 ]
Zhou, Jingming [2 ]
Zhu, Xifang [2 ]
Zhang, Ying [2 ]
Liang, Chao [2 ]
Zhang, Gaiping [1 ,2 ,3 ,4 ]
机构：
[1] Henan Longhu Modern Immun Lab, Zhengzhou, Henan, Peoples R China
[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China
[3] Peking Univ, Coll Agr, Beijing, Peoples R China
[4] Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China
来源：
VIROLOGY | 2023年 / 586卷
关键词：
Varicella-zoster virus; Glycoprotein E; Immunochromatographic strip; Antigen detection; HERPES-SIMPLEX-VIRUS; PATHOGENESIS; INFECTIONS; VACCINE;
D O I：
10.1016/j.virol.2023.07.008
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Varicella-zoster virus (VZV) is a highly infectious DNA virus that can cause varicella (chickenpox) and herpes zoster (HZ). A simple, sensitive and specific detection method is desirable for the VZV infection. In this study, VZV gE protein, expressed in CHO cells, was used to immunize BALB/c mice for the generation of monoclonal antibodies (mAbs). For the first time, we developed a colloidal gold-based immunochromatographic strip for rapid detection of VZV using a pair of mAbs against gE protein. The limit of detection (LOD) of the strip was 30 ng mL-1 of purified VZV gE antigen, and it could specifically test VZV without cross-reactivity with Enterovirus 71 (EV-71), Herpes simplex virus 1 (HSV-1) and Herpes simplex virus 2 (HSV-2). The coincidence rate between the strip and commercial real-time PCR diagnostic kit was 100% using vesicle as the clinical sample. Our strip provided a technical support for rapid and specific detection of VZV.
引用
收藏
页码：35 / 42
页数：8
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