Rapid detection of varicella-zoster virus based on an immunochromatographic strip

被引：3

作者：

Wang, Aiping ^{[1
,2
]}

Niu, Yan ^{[2
]}

Zhao, Jianguo ^{[1
,3
]}

Liu, Hongliang ^{[1
,2
]}

Ding, Peiyang ^{[1
,2
]}

Chen, Yumei ^{[2
]}

Zhou, Jingming ^{[2
]}

Zhu, Xifang ^{[2
]}

Zhang, Ying ^{[2
]}

Liang, Chao ^{[2
]}

Zhang, Gaiping ^{[1
,2
,3
,4
]}

机构：

[1] Henan Longhu Modern Immun Lab, Zhengzhou, Henan, Peoples R China

[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China

[3] Peking Univ, Coll Agr, Beijing, Peoples R China

[4] Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China

来源：

VIROLOGY | 2023年 / 586卷

关键词：

Varicella-zoster virus; Glycoprotein E; Immunochromatographic strip; Antigen detection; HERPES-SIMPLEX-VIRUS; PATHOGENESIS; INFECTIONS; VACCINE;

D O I：

10.1016/j.virol.2023.07.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Varicella-zoster virus (VZV) is a highly infectious DNA virus that can cause varicella (chickenpox) and herpes zoster (HZ). A simple, sensitive and specific detection method is desirable for the VZV infection. In this study, VZV gE protein, expressed in CHO cells, was used to immunize BALB/c mice for the generation of monoclonal antibodies (mAbs). For the first time, we developed a colloidal gold-based immunochromatographic strip for rapid detection of VZV using a pair of mAbs against gE protein. The limit of detection (LOD) of the strip was 30 ng mL-1 of purified VZV gE antigen, and it could specifically test VZV without cross-reactivity with Enterovirus 71 (EV-71), Herpes simplex virus 1 (HSV-1) and Herpes simplex virus 2 (HSV-2). The coincidence rate between the strip and commercial real-time PCR diagnostic kit was 100% using vesicle as the clinical sample. Our strip provided a technical support for rapid and specific detection of VZV.

引用

页码：35 / 42

页数：8

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