Characteristics of phenotypic antibiotic resistance of Helicobacter pylori and its correlation with genotypic antibiotic resistance: A retrospective study in Ningxia

被引：12

作者：

Hu, Shengjuan ^{[1
,14
]}

Zhou, Yan ^{[1
]}

Deng, Yanhong ^{[1
]}

Bo, Yang ^{[1
]}

Chen, Xianmei ^{[1
]}

Yang, Wei ^{[2
]}

Shi, Ruichun ^{[3
]}

Zhao, Wei ^{[1
]}

Hou, Zhanbing ^{[4
]}

Hu, Jianping ^{[5
]}

Liu, Jianguo

Zhang, Xilong

Yong, Heli ^{[8
]}

Wang, Ping ^{[9
]}

Li, Fei ^{[9
]}

Qi, Hailong

Wang, Xiaoyun

Jin, Lijuan ^{[2
]}

Cui, Ting ^{[6
]}

Yong, Haijiang ^{[3
]}

Li, Xue ^{[1
]}

Yang, Bin ^{[7
]}

Yu, Yuehua ^{[5
]}

Ma, Bin ^{[5
]}

Fu, Lei ^{[10
]}

Wang, Xuemei ^{[6
]}

Ma, Zhen ^{[9
]}

Tang, Na ^{[11
]}

You, Yanjie ^{[1
]}

Guo, Jianyang ^{[1
]}

Yu, Xiaobing ^{[1
]}

Yao, Li ^{[1
]}

Gao, Ruiping ^{[1
]}

Li, Yanling ^{[1
]}

Xin, Ruijuan ^{[1
]}

Liu, Jianfang ^{[1
]}

Cao, Zhenzi ^{[1
]}

Li, Hongliang ^{[1
]}

Ma, Linke ^{[1
]}

Ma, Shoucheng ^{[1
]}

Cao, Ying ^{[1
]}

Tang, Yuanyuan ^{[1
]}

Liu, Jun ^{[1
]}

Hao, Qian ^{[1
]}

Li, Xiaofei ^{[1
]}

Li, Xuemei ^{[1
]}

Mu, Rui ^{[1
]}

Niu, Min ^{[1
]}

Su, Xiaoming ^{[1
]}

Gao, Hengjun ^{[12
,13
,15
]}

机构：

[1] Ningxia Med Univ, Peoples Hosp Ningxia Hui Autonomous Reg, Affiliated Peoples Hosp Autonomous Reg, Yinchuan, Peoples R China

[2] Ningxia Integrated Tradit Chinese & Western Med Ho, Yinchuan, Peoples R China

[3] Wuzhong Peoples Hosp, Dept Gastroenterol, Wuzhong, Peoples R China

[4] Yanchi Peoples Hosp, Dept Gastroenterol, Yanchi, Peoples R China

[5] Yinchuan First Peoples Hosp, Dept Gastroenterol, Yinchuan, Peoples R China

[6] Zhongwei Peoples Hosp, Dept Gastroenterol, Zhongwei, Peoples R China

[7] Peoples Hosp Pingluo Cty, Dept Gastroenterol, Pingluo, Peoples R China

[8] Guyuan Yuanzhou Dist Peoples Hosp, Dept Gastroenterol, Guyuan, Peoples R China

[9] Fifth Peoples Hosp Ningxia Hui Autonomous Reg, Dept Gastroenterol, Yinchuan, Peoples R China

[10] Second Peoples Hosp Shizuishan, Dept Gastroenterol, Shizuishan, Peoples R China

[11] Shizuishan First Peoples Hosp, Dept Gastroenterol, Shizuishan, Peoples R China

[12] Tongji Univ, Tongji Hosp, Sch Med, Shanghai, Peoples R China

[13] Tongji Univ, Inst Digest Dis, Sch Med, Shanghai, Peoples R China

[14] Ningxia Med Univ, Peoples Hosp Ningxia Hui Autonomous Reg, Affiliated Peoples Hosp Autonomous Reg, 301 Zhengyuan North St, Yinchuan 750001, Ningxia Hui Aut, Peoples R China

[15] Tongji Univ, Tongji Hosp, Sch Med, Shanghai, Peoples R China

来源：

HELICOBACTER | 2023年 / 28卷 / 03期

关键词：

antibiotic resistance; eradication therapy; gastric disease; genotype; Helicobacter pylori; phenotype; GASTRIC-CANCER; CLARITHROMYCIN RESISTANCE; INFECTION; POPULATION; MUTATIONS; STRAINS;

D O I：

10.1111/hel.12960

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Geographic differences exist in the antibiotic resistance patterns of Helicobacter pylori. Personalized treatment regimens based on local or individual resistance data are essential. We evaluated the current status of H. pylori resistance in Ningxia, analyzed resistance-related factors, and assessed the concordance of phenotypic and genotypic resistance.Methods: Strains were isolated from the gastric mucosa of patients infected with H. pylori in Ningxia and relevant clinical information was collected. Phenotypic antibiotic susceptibility assays (Kirby-Bauer disk diffusion) and antibiotic resistance gene detection (Sanger sequencing) were performed.Results: We isolated 1955 H. pylori strains. The resistance rates of H. pylori to amoxicillin, levofloxacin, clarithromycin, and metronidazole were 0.9%, 42.4%, 40.4%, and 94.2%, respectively. Only five tetracycline-resistant and one furazolidone-resistant strain were identified. Overall, 3.3% of the strains were sensitive to all six antibiotics. Multidrug-resistant strains accounted for 22.9%, of which less than 20% were from Wuzhong. Strains isolated from women and patients with nonulcerative disease had higher rates of resistance to levofloxacin and clarithromycin. Higher rates of resistance to metronidazole, levofloxacin, and clarithromycin were observed in the older age group than in the younger age group. The kappa coefficients of phenotypic resistance and genotypic resistance for levofloxacin and clarithromycin were 0.830 and 0.809, respectively, whereas the remaining antibiotics showed poor agreement.Conclusion: H. pylori antibiotic resistance is severe in Ningxia. Therefore, furazolidone, amoxicillin, and tetracycline are better choices for the empirical therapy of H. pylori infection in this region. Host sex, age, and the presence of ulcerative diseases may affect antibiotic resistance of the bacteria. Personalized therapy based on genetic testing for levofloxacin and clarithromycin resistance may be a future direction for the eradication therapy of H. pylori infection in Ningxia.

引用

页数：11

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