Integrative multi-omics unravels the amelioration effects of Zanthoxylum bungeanum Maxim. on non-alcoholic fatty liver disease

被引：17

作者：

Huang, Xuemei ^{[1
]}

Yuan, Zhihua ^{[1
]}

Liu, Xiaojing ^{[1
]}

Wang, Zhijia ^{[1
]}

Lu, Jihui ^{[1
]}

Wu, Linying ^{[1
]}

Lin, Xiaoyu ^{[1
]}

Zhang, Yaozhi ^{[1
]}

Pi, Wenmin ^{[1
]}

Cai, Desheng ^{[1
]}

Chu, Fuhao ^{[1
]}

Wang, Penglong ^{[1
]}

Lei, Haimin ^{[1
]}

机构：

[1] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 102488, Peoples R China

来源：

PHYTOMEDICINE | 2023年 / 109卷

基金：

中国国家自然科学基金;

关键词：

Zanthoxylum bungeanum Maxim; Non-alcoholic fatty liver; Multi-omics; Liver transcriptomic; Gut microbiota; Non-targeted metabonomic; HEPATIC STEATOSIS; EXTRACT; DIET; MICE; HYPEROSIDE; METABOLISM; STRESS; OIL;

D O I：

10.1016/j.phymed.2022.154576

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: The effect of Zanthoxylum bungeanum Maxim. (ZBM) on anti-obesity, lipid-lowering and liver pro-tection has been identified, but the effect on the development of NAFLD induced by high-fat diet remains unclear. Purpose: To evaluate the alleviation effect of ZBM on NAFLD in vivo and explore the mechanisms by analyzing the liver transcriptome, microbiota and fecal metabolites. Methods: NAFLD model was induced in C57BL/6J mice by feeding with high-fat diet (HFD). The potential mechanism of ZBM in improving NAFLD was studied by liver transcriptome analysis, real-time PCR, immuno-fluorescence, 16s rRNA sequencing and non-targeted metabonomics. Results: ZBM has alleviation effects on HFD-induced NAFLD. The liver transcriptome, real-time PCR and immunofluorescence analysis showed that ZBM could efficiently regulate fatty acid and cholesterol metabolism. The 16S rRNA sequencing and LC-MS based metabonomic demonstrated that ZBM could rebalance gut micro-biota dysbiosis and regulate metabolic profiles in HFD-induced NAFLD mice. Spearman correlation analysis revealed a strong correlation between gut microbiota and biochemical, pathological indexes and differential metabolic biomarkers. Conclusion: ZBM ameliorates HFD-induced NAFLD by regulating fatty acid and cholesterol metabolism, gut microbiota and metabolic profile.

引用

页数：13

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