Correlation of brittle matrix powder properties to aerodynamic performance of inhaled nintedanib made by thin-film freezing

Nintedanib is a drug approved to treat idiopathic pulmonary fibrosis (IPF) and all chronic progressive fibrosing interstitial lung diseases. Delivery by dry powder inhalation appears to be a potential route to improve efficacy and safety over the oral administration. Inhaled nintedanib powders (TFF-NIN) were produced by thin-film freezing (TFF). The correlation between the physical properties and the aerodynamic performance was evaluated by multivariate correlation analysis presented by the correlation coefficient of each parameter along with its statistical significance. Drug loading and excipients were designed to evaluate the correlation. X-ray powder diffraction (XRPD) and scanning electron microscopy (SEM) demonstrated that TFF produced all formulations of the TFF-NIN as amorphous nintedanib and brittle matrix structures. The physical characteristics of brittle matrix powders, including density, specific surface area (SSA), geometric particle size (Dv50), fiowability, powder dispersion, powder deformation, water sorption and moisture content were studied for the correlation with aerodynamic performance. The TFF-NIN powders with high SSA, high fiowability, small particle size, highdegree brittle fracture and low moisture content displayed significantly high aerodynamic performance with correlation coefficients over 0.8 (p < 0.05). A higher drug loading decreased the characteristics of brittle matrix powders by increasing particle size and reducing SSA and fiowability, which also reduced aerodynamic performance. A higher amount of leucine improved the fiowability, SSA, brittle fracture, and aerodynamic performance. In conclusion, the TFF-NIN powders, which exhibited amorphous and brittle matrix morphology, had physical characteristics including SSA, Dv50, fiowability, brittle fracture, and moisture content that significantly correlated with aerodynamic performance.