Organic fluorescent probes for live-cell super-resolution imaging

被引:8
|
作者
Duan, Xinxin [1 ]
Zhang, Meng [1 ]
Zhang, Yu-Hui [1 ]
机构
[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, MoE Key Lab Biomed Photon, Adv Biomed Imaging Facil,Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Super-resolution imaging; Organic fluorescent dyes; Live-cell imaging; Cell-impermeable organic probes; GENERAL-METHOD; FLUOROGENIC PROBES; RESOLUTION LIMIT; STED MICROSCOPY; LONG-TERM; IN-VIVO; MITOCHONDRIA; NANOSCALE; RECONSTRUCTION; FLUOROPHORES;
D O I
10.1007/s12200-023-00090-3
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future.
引用
收藏
页数:12
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