DNA/protein binding and anticancer activity of ruthenium (II) arene complexes based on quinoline dipyrrin

被引：12

作者：

Singh, Nikhil Kumar ^{[1
]}

Kumar, Yogesh ^{[1
]}

Paitandi, Rajendra Prasad ^{[1
]}

Tiwari, Rajan Kumar ^{[2
]}

Kumar, Ajay ^{[2
]}

Pandey, Daya Shankar ^{[1
]}

机构：

[1] Banaras Hindu Univ, Inst Sci, Dept Chem, Varanasi 221005, UP, India

[2] Banaras Hindu Univ, Inst Sci, Dept Zool, Varanasi 221005, UP, India

来源：

INORGANICA CHIMICA ACTA | 2023年 / 545卷

关键词：

Ru(II)-arene complexes; DNA/protein interactions; Anticancer activity; DFT calculations; SANDWICH RU(II)-ARENE COMPLEXES; TRANSITION-METAL-COMPLEXES; IN-VITRO; DNA-BINDING; MOLECULAR-STRUCTURE; IR(III) COMPLEXES; PROTEIN-BINDING; LYMPHOMA-CELLS; ANTITUMOR; LIGANDS;

D O I：

10.1016/j.ica.2022.121241

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Arene ruthenium complexes [(eta(6)-arene)Ru(L)Cl] (eta(6)-arene = benzene, R1; p-cymene, R2) containing the chelating ligand (2-chloro-3-(di(1H-pyrrol-2-yl)methyl)quinoline) (L) have been synthesized and carefully characterized by various studies (H-1 and C-13 NMR, IR, ESI-MS, UV-vis). The structure of R1 has been verified by X-ray single crystal analyses. Binding of the complexes with calf thymus DNA (CT-DNA) has been investigated by absorption titration, ethidium bromide (EB) displacement and viscosity measurements. Experimental binding constants (5.1 x 10(4) M-1, R1; 5.7 x 10(4) M-1, R2) suggested appreciable bonding of the complexes with CT-DNA. Fluorescence, synchronous and 3D fluorescence spectroscopic studies showed that complexes strongly bind with bovine serum albumin. Intercalative interaction of the complexes with DNA has been further supported by DFT studies. Our studies have shown potential anticancer activity of both the complexes R1 and R2 against T cell lymphoma. Among these complexes R2 showed better anticancer activity relative to R1 (IC50, R1, 30 mu M and R2, 20 mu M).

引用

页数：11

共 87 条

[1] In vitro and in vivo activity and cross resistance profiles of novel ruthenium (II) organometallic arene complexes in human ovarian cancer [J].