Transcriptional networks orchestrating red and pink testa color in peanut

被引：24

作者：

Ahmad, Naveed ^{[1
,2
,3
]}

Zhang, Kun ^{[1
,2
,4
]}

Ma, Jing ^{[1
,2
,5
]}

Yuan, Mei ^{[6
]}

Zhao, Shuzhen ^{[1
,2
]}

Wang, Mingqing ^{[6
]}

Deng, Li ^{[7
]}

Ren, Li ^{[7
]}

Gangurde, Sunil S. ^{[8
,9
]}

Pan, Jiaowen ^{[1
,2
]}

Ma, Changle ^{[5
]}

Li, Changsheng ^{[1
,2
]}

Guo, Baozhu ^{[8
,9
]}

Wang, Xingjun ^{[1
,2
,5
]}

Li, Aiqin ^{[1
,2
]}

Zhao, Chuanzhi ^{[1
,2
,5
]}

机构：

[1] Shandong Acad Agr Sci, Inst Biotechnol, Inst crop germplasm resources, Jinan 250100, Peoples R China

[2] Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China

[3] Shanghai Jiao Tong Univ, Joint Ctr Single Cell Biol, Sch Agr & Biol, Shanghai 200240, Peoples R China

[4] Shandong Agr & Engn Univ, Coll Agr Sci & Technol, Jinan 250100, Peoples R China

[5] Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China

[6] Shandong Peanut Res Inst, Qingdao 266199, Shandong, Peoples R China

[7] Kaifeng Acad Agr & Forestry, Kaifeng 475008, Peoples R China

[8] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA

[9] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA

来源：

BMC PLANT BIOLOGY | 2023年 / 23卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Peanut; Testa color; Anthocyanin biosynthesis; flavonoids; Bulk RNA-seq (BR-seq); Transcription factors; FLAVONOID BIOSYNTHETIC-PATHWAY; SEED COAT DEVELOPMENT; ANTHOCYANIN BIOSYNTHESIS; ANALYSIS REVEALS; MOLECULAR ANALYSIS; CORRELATED GENES; DOWN-REGULATION; SKIN COLOR; EXPRESSION; ARABIDOPSIS;

D O I：

10.1186/s12870-023-04041-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundTesta color is an important trait of peanut (Arachis hypogaea L.) which is closely related with the nutritional and commercial value. Pink and red are main color of peanut testa. However, the genetic mechanism of testa color regulation in peanut is not fully understood. To elucidate a clear picture of peanut testa regulatory model, samples of pink cultivar (Y9102), red cultivar (ZH12), and two RNA pools (bulk red and bulk pink) constructed from F-4 lines of Y9102 x ZH12 were compared through a bulk RNA-seq approach.ResultsA total of 2992 differential expressed genes (DEGs) were identified among which 317 and 1334 were up-regulated and 225 and 1116 were down-regulated in the bulk red-vs-bulk pink RNA pools and Y9102-vs-ZH12, respectively. KEGG analysis indicates that these genes were divided into significantly enriched metabolic pathways including phenylpropanoid, flavonoid/anthocyanin, isoflavonoid and lignin biosynthetic pathways. Notably, the expression of the anthocyanin upstream regulatory genes PAL, CHS, and CHI was upregulated in pink and red testa peanuts, indicating that their regulation may occur before to the advent of testa pigmentation. However, the differential expression of down-stream regulatory genes including F3H, DFR, and ANS revealed that deepening of testa color not only depends on their gene expression bias, but also linked with FLS inhibition. In addition, the down-regulation of HCT, IFS, HID, 7-IOMT, and I2'H genes provided an alternative mechanism for promoting anthocyanin accumulation via perturbation of lignin and isoflavone pathways. Furthermore, the co-expression module of MYB, bHLH, and WRKY transcription factors also suggested a fascinating transcriptional activation complex, where MYB-bHLH could utilize WRKY as a co-option during the testa color regulation by augmenting anthocyanin biosynthesis in peanut.ConclusionsThese findings reveal candidate functional genes and potential strategies for the manipulation of anthocyanin biosynthesis to improve peanut varieties with desirable testa color.

引用

页数：20

共 74 条

[1] Bulk RNA-Seq Analysis Reveals Differentially Expressed Genes Associated with Lateral Branch Angle in Peanut [J].