A New Model of Esophageal Cancers by Using a Detergent-Free Decellularized Matrix in a Perfusion Bioreactor

被引:2
作者
Brennan, Jordan [1 ]
Lu, Michael L. [2 ,3 ]
Kang, Yunqing [1 ,2 ,3 ]
机构
[1] Florida Atlantic Univ, Coll Engn & Comp Sci, Dept Ocean & Mech Engn, Boca Raton, FL 33431 USA
[2] Florida Atlantic Univ, Charles E Schmidt Coll Med, Dept Biomed Sci, Boca Raton, FL 33431 USA
[3] Florida Atlantic Univ, Fac Integrat Biol PhD Program, Dept Biol Sci, Boca Raton, FL 33431 USA
来源
BIOENGINEERING-BASEL | 2023年 / 10卷 / 01期
基金
美国国家卫生研究院;
关键词
decellularization; esophageal cancer; dynamic culture; cancer model;
D O I
10.3390/bioengineering10010096
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The lack of physiologically relevant human esophageal cancer models has as a result that many esophageal cancer studies are encountering major bottleneck challenges in achieving breakthrough progress. To address the issue, here we engineered a 3D esophageal tumor tissue model using a biomimetic decellularized esophageal matrix in a customized bioreactor. To obtain a biomimetic esophageal matrix, we developed a detergent-free, rapid decellularization method to decellularize porcine esophagus. We characterized the decellularized esophageal matrix (DEM) and utilized the DEM for the growth of esophageal cancer cell KYSE30 in well plates and the bioreactor. We then analyzed the expression of cancer-related markers of KYSE30 cells and compared them with formalin-fixed, paraffin-embedded (FFPE) esophageal squamous cell carcinoma (ESCC) tissue biospecimens. Our results show that the detergent-free decellularization method preserved the esophageal matrix components and effectively removed cell nucleus. KYSE30 cancer cells proliferated well on and inside the DEM. KYSE30 cells cultured on the DEM in the dynamic bioreactor show different cancer marker expressions than those in the static well plate, and also share some similarities to the FFPE-ESCC biospecimens. These findings built a foundation with potential for further study of esophageal cancer behavior in a biomimetic microenvironment using this new esophageal cancer model.
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页数:17
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