Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

被引：16

作者：

Arsene, Mbarga Manga Joseph ^{[1
,2
]}

Viktorovna, Podoprigora Irina ^{[1
,2
]}

Alla, Marukhlenko ^{[3
]}

Mariya, Morozova ^{[3
]}

Nikolaevitch, Senyagin Alexander ^{[1
,2
]}

Davares, Anyutoulou Kitio Linda ^{[1
]}

Yurievna, Mumrova Evgenia ^{[2
]}

Rehailia, Manar ^{[4
]}

Gabin, Ada Arsene ^{[5
]}

Alekseevna, Kulikova A. ^{[6
]}

Vyacheslavovna, Yashina Natalia ^{[1
]}

Vladimirovna, Zhigunova Anna ^{[1
]}

Svetlana, Orlova ^{[7
]}

Milana, Das ^{[1
]}

机构：

[1] Peoples Friendship Univ Russia RUDN Univ, Med Inst, Dept Microbiol VS Kiktenko, Moscow, Russia

[2] Peoples Friendship Univ Russia RUDN Univ, Res Inst Mol & Cellular Med, Moscow, Russia

[3] Peoples Friendship Univ Russia RUDN Univ, Med Inst, Dept Pharmaceut & Toxicol Chem, Moscow, Russia

[4] Peoples Friendship Univ Russia RUDN Univ, Agr Inst, Dept Agrobiotechnol, Moscow, Russia

[5] Peoples Friendship Univ Russia RUDN Univ, Med Inst, Dept Traumatol & Orthoped, Moscow, Russia

[6] Peoples Friendship Univ Russia RUDN Univ, Med Inst, Dept Oral & Maxillofacial Surg, Moscow, Russia

[7] Peoples Friendship Univ Russia RUDN Univ, Med Inst, Dept Dietet & Clin Nutritiol, Moscow, Russia

来源：

VETERINARY WORLD | 2023年 / 16卷 / 01期

关键词：

Aloe vera; antifungal; antimicrobials; green synthesis; silver nanoparticles; GREEN SYNTHESIS; BARK;

D O I：

10.14202/vetworld.2023.18-26

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Background and Aim: Resistance to antifungal agents is a serious public health concern that has not been investigated enough because most studies on antimicrobials are dedicated to antibacterial resistance. This study aimed to synthesize silver nanoparticles (AgNPs) using Aloe vera extract, and to assess its antifungal activity against Candida albicans.Materials and Methods: Silver nanoparticles were synthesized by reducing Ag nitrate with aqueous A. vera extracts. Physicochemical properties of synthesized AgNPs were determined by ultraviolet-visible spectrophotometry, photon cross-correlation spectroscopy, energy-dispersive X-ray fluorescence spectrometry, X-ray diffraction analysis, and Fourier-transform infrared spectroscopy. An antifungal investigation was performed against four clinical C. albicans (C1, C2, C3, and C4) and a reference strain, C. albicans ATCC 10321.Results: Cubic AgNPs with a mean X50 hydrodynamic diameter of 80.31 +/- 10.03 nm were successfully synthesized. These AgNPs exhibited maximum absorbance at 429.83 nm, and X-ray fluorescence (XRF) confirmed the presence of Ag in AgNPs solution by a characteristic peak in the spectrum at the Ag K alpha line of 22.105 keV. Infrared spectra for AgNPs and A. vera extract indicated that the compounds present in the extract play an essential role in the coating/capping of synthesized AgNPs. Different concentrations (200, 100, 50, 25, 10, and 5 mu g/mL) of AgNPs were tested. The antifungal activity was shown to be dose-dependent with inhibition zones ranging from 10 mm to 22 mm against C. albicans ATCC 10231, 0 mm to 15 mm against C1, 0 mm to 16 mm against C2 and C3, and 0 mm to 14 mm for C4. Minimum inhibitory concentration ranged from 16 mu g/mL to 32 mu g/mL against clinical C. albicans (C1, C2, C3, and C4) and was 4 mu g/mL against C. albicans ATCC 10231.Conclusion: This study showed the ability of A. vera to serve as an efficient reducing agent for the biogenic synthesis of AgNPs with excellent antifungal activity.

引用

页码：18 / 26

页数：9

共 42 条

[1] Optimization of Ethanolic Extraction of Enantia chloranta Bark, Phytochemical Composition, Green Synthesis of Silver Nanoparticles, and Antimicrobial Activity [J].