Transcriptomic analysis of granulosa cells in patients with endometriosis-related infertility: identification of potential molecular mechanisms

被引：0
作者：
Zhang, Jian [1 ]
Zhang, Yu [1 ]
Luo, Jia-Li [1 ]
Yu, Lin [1 ]
Gao, Min-Zhi [2 ,4 ]
Sun, Zhao-Gui [1 ,3 ]
机构：
[1] Fudan Univ, Shanghai Inst Biomed & Pharmaceut Technol, Shanghai Engn Res Ctr Reprod Hlth Drug & Devices, NHC Key Lab Reprod Regulat,Sch Basic Med Sci, Shanghai 200237, Peoples R China
[2] Shanghai Jiao Tong Univ, Shanghai Key Lab Assisted Reprod & Reprod Genet, Ctr Reprod Med, Renji Hosp,Sch Med, Shanghai 200135, Peoples R China
[3] Fudan Univ, Shanghai Inst Biomed & Pharmaceut Technol, Med Sch, 2140 Xietu Rd, Shanghai 200237, Peoples R China
[4] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Shanghai Key Lab Assisted Reprod & Reprod Genet,Ct, 845 Lingshan Rd, Shanghai 200135, Peoples R China
来源：
REPRODUCTIVE AND DEVELOPMENTAL MEDICINE | 2023年 / 7卷 / 04期
关键词：
Endometriosis; Female infertility; Granulosa cells; RNA sequencing; IN-VITRO FERTILIZATION; FOLLICULAR-FLUID; EXPRESSION; OOCYTE; ACTIVATION; REDUCTION; QUALITY; MARKERS; WOMEN;
D O I：
10.1097/RD9.0000000000000075
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective:To investigate the impact of endometriosis (EMS) on granulosa cell function and elucidate the molecular mechanisms involved.Methods:RNA sequencing, differential expression analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, gene set enrichment analysis, protein-protein interaction analysis, and RT-qPCR were employed to assess the effects of EMS on granulosa cell function.Results:The results revealed significant differences in gene expression between the EMS and control groups, including genes related to immune regulatory functions and ferroptosis. Hub gene modules and hub genes were identified, including those related to cell cycle and immune and inflammatory pathways. RT-qPCR revealed significant upregulation of the hub genes CCL3 and IL1B in granulosa cells of patients with EMS.Conclusion:The results of RNA sequencing demonstrated that EMS induces significant transcriptional alterations in granulosa cells of affected patients. These findings provide important insights into the diagnosis and treatment of EMS and highlight the importance of further investigation of CCL3 and IL1B as potential biomarkers for EMS.
引用
收藏
页码：193 / 202
页数：10
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