Borrelia burgdorferi Outer Surface Protein C Is Not the Sole Determinant of Dissemination in Mammals

被引:4
作者
Mukherjee, Priyanka G. [1 ]
Liveris, Dionysios [1 ]
Hanincova, Klara [1 ]
Iyer, Radha [1 ]
Wormser, Gary P. [2 ]
Huang, Weihua [1 ]
Schwartz, Ira [1 ,2 ]
机构
[1] New York Med Coll, Dept Pathol Microbiol & Immunol, Valhalla, NY 10595 USA
[2] New York Med Coll, Dept Med, Valhalla, NY 10595 USA
关键词
Borrelia burgdorferi; Lyme disease; OspC; dissemination; LYME-DISEASE SPIROCHETE; SENSU-STRICTO; HEMATOGENOUS DISSEMINATION; PLASMID CONTENT; READ ALIGNMENT; REVA ANTIGEN; OSPC; INFECTION; MEMBRANE; GENOTYPE;
D O I
10.1128/iai.00456-22
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lyme disease in the United States is most often caused by Borrelia burgdorferi sensu stricto. After a tick bite, the patient may develop erythema migrans at that site. If hematogenous dissemination occurs, the patient may then develop neurologic manifestations, carditis, or arthritis. Host-pathogen interactions include factors that contribute to hematogenous dissemination to other body sites. Outer surface protein C (OspC), a surface-exposed lipoprotein of B. burgdorferi, is essential during the early stages of mammalian infection. There is a high degree of genetic variation at the ospC locus, and certain ospC types are more frequently associated with hematogenous dissemination in patients, suggesting that OspC may be a major contributing factor to the clinical outcome of B. burgdorferi infection. In order to evaluate the role of OspC in B. burgdorferi dissemination, ospC was exchanged between B. burgdorferi isolates with different capacities to disseminate in laboratory mice, and these strains were then tested for their ability to disseminate in mice. The results indicated that the ability of B. burgdorferi to disseminate in mammalian hosts does not depend on OspC alone. The complete genome sequences of two closely related strains of B. burgdorferi with differing dissemination phenotypes were determined, but a specific genetic locus that could explain the differences in the phenotypes could not be definitively identified. The animal studies performed clearly demonstrated that OspC is not the sole determinant of dissemination. Future studies of the type described here with additional borrelial strains will hopefully clarify the genetic elements associated with hematogenous dissemination. Lyme disease in the United States is most often caused by Borrelia burgdorferi sensu stricto. After a tick bite, the patient may develop erythema migrans at that site.
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共 62 条
[1]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[2]   Borrelia burgdorferi RevA Antigen Binds Host Fibronectin [J].
Brissette, Catherine A. ;
Bykowski, Tomasz ;
Cooley, Anne E. ;
Bowman, Amy ;
Stevenson, Brian .
INFECTION AND IMMUNITY, 2009, 77 (07) :2802-2812
[3]   Biodiversity of Borrelia burgdorferi Strains in Tissues of Lyme Disease Patients [J].
Brisson, Dustin ;
Baxamusa, Nilofer ;
Schwartz, Ira ;
Wormser, Gary P. .
PLOS ONE, 2011, 6 (08)
[4]   Borrelia burgdorferi RevA Significantly Affects Pathogenicity and Host Response in the Mouse Model of Lyme Disease [J].
Byram, Rebecca ;
Gaultney, Robert A. ;
Floden, Angela M. ;
Hellekson, Christopher ;
Stone, Brandee L. ;
Bowman, Amy ;
Stevenson, Brian ;
Johnson, Barbara J. B. ;
Brissette, Catherine A. .
INFECTION AND IMMUNITY, 2015, 83 (09) :3675-3683
[5]   The RpoS Gatekeeper in Borrelia burgdorferi: An Invariant Regulatory Scheme That Promotes Spirochete Persistence in Reservoir Hosts and Niche Diversity [J].
Caimano, Melissa J. ;
Groshong, Ashley M. ;
Belperron, Alexia ;
Mao, Jialing ;
Hawley, Kelly L. ;
Luthra, Amit ;
Graham, Danielle E. ;
Earnhart, Christopher G. ;
Marconi, Richard T. ;
Bockensted, Linda K. ;
Bleyins, Jon S. ;
Radolf, Justin D. .
FRONTIERS IN MICROBIOLOGY, 2019, 10
[6]   Interaction of the Lyme disease spirochete with its tick vector [J].
Caimano, Melissa J. ;
Drecktrah, Dan ;
Kung, Faith ;
Samuels, D. Scott .
CELLULAR MICROBIOLOGY, 2016, 18 (07) :919-927
[7]   Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival [J].
Caine, Jennifer A. ;
Lin, Yi-Pin ;
Kessler, Julie R. ;
Sato, Hiromi ;
Leong, John M. ;
Coburn, Jenifer .
CELLULAR MICROBIOLOGY, 2017, 19 (12)
[8]   Outer Surface Protein OspC Is an Antiphagocytic Factor That Protects Borrelia burgdorferi from Phagocytosis by Macrophages [J].
Carrasco, Sebastian E. ;
Troxell, Bryan ;
Yang, Youyun ;
Brandt, Stephanie L. ;
Li, Hongxia ;
Sandusky, George E. ;
Condon, Keith W. ;
Serezani, C. Henrique ;
Yang, X. Frank .
INFECTION AND IMMUNITY, 2015, 83 (12) :4848-4860
[9]   Borrelia burgdorferi RevA antigen is a surface-exposed outer membrane protein whose expression is regulated in response to environmental temperature and pH [J].
Carroll, JA ;
El-Hage, N ;
Miller, JC ;
Babb, K ;
Stevenson, B .
INFECTION AND IMMUNITY, 2001, 69 (09) :5286-5293
[10]   A bacterial genome in flux:: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi [J].
Casjens, S ;
Palmer, N ;
van Vugt, R ;
Huang, WM ;
Stevenson, B ;
Rosa, P ;
Lathigra, R ;
Sutton, G ;
Peterson, J ;
Dodson, RJ ;
Haft, D ;
Hickey, E ;
Gwinn, M ;
White, O ;
Fraser, CM .
MOLECULAR MICROBIOLOGY, 2000, 35 (03) :490-516