HPLC Analysis of Polyphenolic Compounds in Lysimachia nummularia L. and Comparative Determination of Antioxidant Capacity

被引：6

作者：

Suciu, Felicia ^{[1
]}

Stoicescu, Iuliana ^{[2
]}

Lupu, Elena Carmen ^{[3
]}

Musuc, Adina Magdalena ^{[4
]}

Popescu, Antoanela ^{[5
]}

Mititelu, Magdalena ^{[6
]}

Rosca, Adrian Cosmin ^{[1
]}

Dumitrescu, Denisa-Elena ^{[7
]}

Badea, Florin Ciprian ^{[8
]}

Caraiane, Aureliana ^{[9
]}

Badea, Victoria ^{[10
]}

机构：

[1] Ovidius Univ Constanta, Fac Pharm, Dept Anal & Qual Control Drugs, Constanta 900470, Romania

[2] Ovidius Univ Constanta, Fac Pharm, Dept Chem & Qual Control Drugs, Constanta 900470, Romania

[3] Ovidius Univ Constanta, Fac Pharm, Dept Math & Informat, Constanta 900470, Romania

[4] Ilie Murgulescu Inst Phys Chem, Bucharest 060021, Romania

[5] Ovidius Univ Constanta, Fac Pharm, Dept Pharmacognosy, Constanta 900470, Romania

[6] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Clin Lab & Food Safety, Bucharest 020956, Romania

[7] Ovidius Univ Constanta, Fac Pharm, Dept Organ Chem, Constanta 900470, Romania

[8] Ovidius Univ Constanta, Fac Dent Med, Dept Dent Med, Constanta 900684, Romania

[9] Ovidius Univ Constanta, Fac Dent Med, Dept Oral Rehabil, Constanta 900684, Romania

[10] Ovidius Univ Constanta, Fac Dent Med, Dept Microbiol & Immunol, Constanta 900684, Romania

来源：

APPLIED SCIENCES-BASEL | 2023年 / 13卷 / 04期

关键词：

Lysimachia; polyphenols; antioxidant activity; trans-resveratrol; 3-O-methylgallic acid; bioactive compounds; EXTRACTS; PRIMULACEAE; FLAVONOIDS; PROFILE; PARTS;

D O I：

10.3390/app13042159

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lysimachia nummularia L. is a perennial herbaceous plant rich in bioactive compounds, which can be utilized for medicinal purposes. The present work aims to analyze the phenolic compounds from different parts of the Lysimachia nummularia L. plant using the HPLC technique: Lysimachiae radix (the root part), Lysimachiae herba (the aerial part), Lysimachiae flores (flowers). In order to determine the phenolic compounds, extraction from the three categories of vegetable products was performed with ethanol 70% (v/v) using three extraction methods: (i) Soxhlet extraction, (ii) maceration and (iii) ultrasonic-assisted extraction. The content of polyphenols was determined by the Folin-Ciocalteu method, and the antioxidant activity was evaluated by the DPPH, ABTS, FRAP and CUPRAC methods. The antioxidant activity was correlated with the content of phenolic compounds in the analyzed extracts. The following phenolic compounds were separated, identified and quantified: 3-O-methylgallic, gallic, ferulic, caffeic, chlorogenic, p-coumaric acids and trans-resveratrol. According to the experimental data, the highest content of total polyphenols was observed in the hydroethanolic extract from Lysimachiae flores (22.10 +/- 1.48 mg gallic acid/g), which also presented remarkable antioxidant activity.

引用

页数：15

共 49 条

[1] EVALUATION OF PHENOLIC COMPOUNDS IN TILIA RUBRA SUBSP CAUCASICA BY HPLC-UV AND HPLC-UV-MS/MS [J].