16S microbiome analysis of microbial communities in distribution centers handling fresh produce

被引:1
|
作者
Townsend, Anna [1 ]
den Bakker, Hendrik C. [2 ]
Mann, Amy [2 ]
Murphy, Claire M. [3 ]
Strawn, Laura K. [3 ]
Dunn, Laurel L. [1 ]
机构
[1] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA
[2] Univ Georgia, Ctr Food Safety, Dept Food Sci & Technol, Griffin, GA USA
[3] Virginia Tech, Dept Food Sci & Technol, Blacksburg, VA USA
关键词
Listeria; built environment; next generation sequencing; food safety; supply chain; FOOD SAFETY; SP-NOV; PSEUDOMONAS-FLUORESCENS; LISTERIA-MONOCYTOGENES; DIVERSITY; PREVALENCE; PATHOGENS; SPOILAGE;
D O I
10.3389/fmicb.2023.1041936
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Little is known about the microbial communities found in distribution centers (DCs), especially in those storing and handling food. As many foodborne bacteria are known to establish residence in food facilities, it is reasonable to assume that DCs handling foods are also susceptible to pathogen colonization. To investigate the microbial communities within DCs, 16S amplicon sequencing was completed on 317 environmental surface sponge swabs collected in DCs (n = 18) across the United States. An additional 317 swabs were collected in parallel to determine if any viable Listeria species were also present at each sampling site. There were significant differences in median diversity measures (observed, Shannon, and Chao1) across individual DCs, and top genera across all reads were Carnobacterium_A, Psychrobacter, Pseudomonas_E, Leaf454, and Staphylococcus based on taxonomic classifications using the Genome Taxonomy Database. Of the 39 16S samples containing Listeria ASVs, four of these samples had corresponding Listeria positive microbiological samples. Data indicated a predominance of ASVs identified as cold-tolerant bacteria in environmental samples collected in DCs. Differential abundance analysis identified Carnobacterium_A, Psychrobacter, and Pseudomonas_E present at a significantly greater abundance in Listeria positive microbiological compared to those negative for Listeria. Additionally, microbiome composition varied significantly across groupings within variables (e.g., DC, season, general sampling location).
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页数:12
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