The Tartary buckwheat bHLH gene ALCATRAZ contributes to silique dehiscence in Arabidopsis thaliana

被引:1
|
作者
Sun, Wenjun [1 ]
Chen, Ying [1 ]
Zeng, Jing [1 ]
Li, Chenglei [1 ]
Yao, Min [1 ]
Liu, Moyang [2 ]
Ma, Zhaotang [4 ,5 ]
Huang, Li [1 ]
Yan, Jun [3 ]
Zhan, Junyi [6 ]
Chen, Hui [1 ]
Bu, Tongliang [1 ]
Tang, Zizong [1 ]
Li, Qingfeng [1 ]
Wu, Qi [1 ]
Hou, Jingfei [1 ]
Huang, Yulin [1 ]
机构
[1] Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Peoples R China
[2] Shanghai Jiao Tong Univ, Joint Ctr Single Cell Biol, Sch Agr & Biol, Shanghai 200240, Peoples R China
[3] Chengdu Univ, Sch Food & Biol Engn, Key Lab Coarse Cereal Proc, Minist Agr & Rural Affairs, Chengdu 610106, Sichuan, Peoples R China
[4] Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Key Lab Major Crop Dis, Chengdu 611130, Peoples R China
[5] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China
[6] Nanjing Agr Univ, Coll Life Sci, Nanjing 210032, Peoples R China
关键词
Silique dehiscence; Lignin; ALC; CELL-WALL; FRUIT DEHISCENCE; SEED DISPERSAL; SPATULA; BIOSYNTHESIS; CARPEL;
D O I
10.1016/j.plantsci.2023.111733
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tartary buckwheat is popular because of its rich nutrients. However, the difficulty in shelling restricts food production. The gene ALCATRAZ (AtALC) plays a key role in silique dehiscence in Arabidopsis thaliana. In this study, an atalc mutant was obtained by CRISPR/Cas9, and a FtALC gene homologous to AtALC was complemented into the atalc mutant to verify its function. Phenotypic observations showed that three atalc mutant lines did not dehiscence, while ComFtALC lines recovered the dehiscence phenotype. The contents of lignin, cellulose, hemicellulose, and pectin in the siliques of all the atalc mutant lines were significantly higher than those in the wild-type and ComFtALC lines. Moreover, FtALC was found to regulate the expression of cell wall pathway genes. Finally, the interaction of FtALC with FtSHP and FtIND was verified by yeast two-hybrid, bimolecular fluorescent complimentary (BIFC) and firefly luciferase completion imaging assays (LCIs). Our findings enrich the silique regulatory network and lay the foundation for the cultivation of easily shelled tartary buckwheat varieties.
引用
收藏
页数:10
相关论文
共 33 条
  • [31] Editing of a Novel Cd Uptake-Related Gene CUP1 Contributes to Reducing Cd Accumulations in Arabidopsis thaliana and Brassica napus
    Yao, Junyu
    Bai, Jiuyuan
    Liu, Sha
    Fu, Jingyan
    Zhang, Ying
    Luo, Tianshun
    Ren, Hongpei
    Wang, Rui
    Zhao, Yun
    CELLS, 2022, 11 (23)
  • [32] Ectopic expression of IiSHP2 from Isatis indigotica Fortune, a PLE-lineage MADS-box gene, influences leaf, floral organ and silique morphology in Arabidopsis thaliana
    Lu, Meng-Xin
    Li, Dian-Zhen
    Pu, Zuo-Qian
    Ma, Yan-Qin
    Huang, Xuan
    Xu, Zi-Qin
    PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2020, 26 (02) : 379 - 389
  • [33] The longer version of Arabidopsis thaliana heat shock protein 18.2 gene terminator contributes to higher expression of stably integrated transgenes in cultured tobacco cells
    Matsui, Takeshi
    Sawada, Kazutoshi
    Takita, Eiji
    Kato, Ko
    PLANT BIOTECHNOLOGY, 2014, 31 (02) : 191 - U113