Disease activity in chronic inflammatory demyelinating polyneuropathy: association between circulating B-cell subsets, cytokine levels, and clinical outcomes

被引:2
|
作者
Acarli, Ayse Nur Ozdag [1 ]
Tuzun, Erdem [2 ]
Sanli, Elif [2 ]
Koral, Gizem [2 ]
Akbayir, Ece [2 ]
Cakar, Arman [1 ]
Sirin, Nermin Gorkem [1 ,3 ]
Soysal, Aysun [3 ]
Aysal, Fikret [3 ]
Durmus, Hacer [1 ]
Parman, Yesim [1 ]
Yilmaz, Vuslat [2 ]
机构
[1] Istanbul Univ, Istanbul Fac Med, Dept Neurol, Neuromuscular Unit, Istanbul, Turkiye
[2] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Neurosci, Istanbul, Turkiye
[3] Bakirkoy Mazhar Osman Mental Hlth & Neurol Dis Edu, Dept Neurol, Istanbul, Turkiye
关键词
chronic inflammatory demyelinating polyneuropathy; B cell; cytokines; miRNA; skin biopsy; epidermal nerve fibers; GUILLAIN-BARRE-SYNDROME; FC-GAMMA-RECEPTOR; T-CELL; CEREBROSPINAL-FLUID; TNF-ALPHA; INTRAVENOUS IMMUNOGLOBULIN; NERVE SOCIETY; REGULATORY T; SKIN BIOPSY; EXPRESSION;
D O I
10.1093/cei/uxad103
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Chronic inflammatory demyelinating polyneuropathy (CIDP), a common and treatable autoimmune neuropathy, is frequently misdiagnosed. The aim of this study is to evaluate the relationship between immunological markers and clinical outcome measures in a mixed cohort of patients with typical CIDP and CIDP variants at different disease stages. Twenty-three typical, 16 multifocal and five distal CIDP patients were included. Twenty-five sex and age-matched healthy controls and 12 patients with Charcot-Marie-Tooth type 1A (CMT1A) disease served as controls. Peripheral B-cell populations were analyzed by flow cytometry. IL6, IL10, TNFA mRNA and mir-21, mir-146a, and mir-155-5p expression levels were evaluated by real-time polymerase chain reaction in peripheral blood mononuclear cells (PBMC) and/or skin biopsy specimens. Results were then assessed for a possible association with clinical disability scores and intraepidermal nerve fiber densities (IENFD) in the distal leg. We detected a significant reduction in naive B cells (P <= 0.001), plasma cells (P <= 0.001) and regulatory B cells (P < 0.05), and an elevation in switched memory B cells (P <= 0.001) in CIDP compared to healthy controls. CMT1A and CIDP patients had comparable B-cell subset distribution. CIDP cases had significantly higher TNFA and IL10 gene expression levels in PBMC compared to healthy controls (P < 0.05 and P <= 0.01, respectively). IENFDs in the distal leg showed a moderate negative correlation with switched memory B-cell ratios (r = -0.51, P < 0.05) and a moderate positive correlation with plasma cell ratios (r = 0.46, P < 0.05). INCAT sum scores showed a moderate positive correlation with IL6 gene expression levels in PBMC (r = 0.54, P < 0.05). Altered B-cell homeostasis and IL10 and TNFA gene expression levels imply chronic antigen exposure and overactivity in the humoral immune system, and seem to be a common pathological pathway in both typical CIDP and CIDP variants.
引用
收藏
页码:65 / 78
页数:14
相关论文
共 50 条
  • [21] Chronic Pain Does Not Impact Baseline Circulating Cytokine Levels in Adults with Sickle Cell Disease
    Karafin, Matthew S.
    Simpson, Pippa
    Field, Joshua J.
    ACTA HAEMATOLOGICA, 2021, 144 (01) : 111 - 116
  • [22] Association between B-cell receptor responsiveness and disease progression in B-cell chronic lymphocytic leukemia: results from single cell network profiling studies
    Cesano, Alessandra
    Perbellini, Omar
    Evensen, Erik
    Chu, Charles C.
    Cioffi, Federica
    Ptacek, Jason
    Damle, Rajendra N.
    Chignola, Roberto
    Cordeiro, James
    Yan, Xiao-jie
    Hawtin, Rachael E.
    Nichele, Ilaria
    Ware, Jodi R.
    Cavallini, Chiara
    Lovato, Ornella
    Zanotti, Roberta
    Rai, Kanti R.
    Chiorazzi, Nicholas
    Pizzolo, Giovanni
    Scupoli, Maria T.
    HAEMATOLOGICA, 2013, 98 (04) : 626 - 634
  • [23] Association between peripheral activated naive and double negative 2 B-cell subsets and clinical parameters in lupus nephritis patients
    Wangriatisak, Kittikorn
    de Vries, Charlotte
    Sharma, Ravi Kumar
    Huang, Wenqi
    Groenwall, Caroline
    Pisitkun, Prapaporn
    Gunnarsson, Iva
    Malmstroem, Vivianne
    Chootong, Patchanee
    Faustini, Francesca
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2025, 101 (01)
  • [24] Altered frequency of peripheral B-cell subsets and their correlation with disease activity in patients with systemic lupus erythematosus: A comprehensive analysis
    Peng, Yanxia
    Guo, Fengbiao
    Liao, Shuzhen
    Liao, Huanjin
    Xiao, Haiyan
    Yang, Lawei
    Liu, Hua-feng
    Pan, Qingjun
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (20) : 12044 - 12053
  • [25] Correlation between orbital immune cell subsets and clinical activity in thyroid eye disease
    Zhang, Mingqiao
    Liang, Liang
    Hu, Hong
    Tang, Chengyang
    Huang, Qian
    Long, Jian
    ANNALES D ENDOCRINOLOGIE, 2025, 86 (04)
  • [26] Elevated circulating adenosine deaminase activity in women with preeclampsia: association with pro-inflammatory cytokine production and uric acid levels
    Giorgi, Vanessa S.
    Witkin, Steven S.
    Bannwart-Castro, Camila F.
    Sartori, Maria Salete
    Romao-Veiga, Mariana
    Borges, Vera Therezinha M.
    Peracoli, Jose Carlos
    Peracoli, Maria Terezinha
    PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH, 2016, 6 (04) : 400 - 405
  • [27] Vitamin B12 Levels in Alzheimer's Disease: Association with Clinical Features and Cytokine Production
    Politis, Antonis
    Olgiati, Paolo
    Malitas, Petros
    Albani, Diego
    Signorini, Alessandra
    Polito, Letizia
    De Mauro, Stefania
    Zisaki, Aikaterini
    Piperi, Christina
    Stamouli, Evangelia
    Mailis, Antonis
    Batelli, Sara
    Forloni, Gianluigi
    De Ronchi, Diana
    Kalofoutis, Anastasios
    Liappas, Ioannis
    Serretti, Alessandro
    JOURNAL OF ALZHEIMERS DISEASE, 2010, 19 (02) : 481 - 488
  • [28] International chronic inflammatory demyelinating polyneuropathy outcome study (ICOS): Protocol of a prospective observational cohort study on clinical and biological predictors of disease course and outcome
    Bunschoten, Carina
    Eftimov, Filip
    van der Pol, W. -Ludo
    Jacobs, Bart C.
    JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM, 2019, 24 (01) : 34 - 38
  • [29] Changes of cytokine levels and T cell surface molecules in patients with chronic hepatitis B and the association with functional cure
    Xu, Zhen
    Lin, Ji-zong
    Zeng, Ying-fu
    Yang, Xiao-hua
    Wu, Zhe-bin
    Hu, Zhao-xia
    Zhao, Qi-yi
    Liu, Jing
    Gao, Zhi-liang
    JOURNAL OF MEDICAL VIROLOGY, 2021, 93 (08) : 4966 - 4974
  • [30] Association between levels of serum and urinary B cell-activating factor and systemic lupus erythematosus disease activity
    Rezazadeh, Maryam
    Jokar, Mohammad Hasan
    Aghili, Seyedeh Mehrnaz
    Mirfeizi, Zahra
    Mahmoudi, Mahmoud
    Morovatdar, Negar
    Hashemzadeh, Kamila
    ARCHIVES OF RHEUMATOLOGY, 2023, 38 (03) : 429 - 440