Synthesis of magnetically recyclable porous cross-linked aggregates of Tramates versicolor MTCC 138 laccase for the efficient removal of pentachlorophenol from aqueous solution

被引:5
作者
Venkataraman, Swethaa [1 ]
Vaidyanathan, Vinoth Kumar [1 ]
机构
[1] SRM Inst Sci & Technol, Dept Biotechnol, Integrated Bioproc Lab, Chennai 603203, Tamil Nadu, India
关键词
Immobilized laccase; Mediator; Surfactant; Rhamnolipid; Pentachlorophenol; TRACE ORGANIC CONTAMINANTS; VERSATILE PEROXIDASE; MESOPOROUS CARBON; WASTE-WATER; IMMOBILIZATION; ENZYME; DEGRADATION; REMEDIATION; SILICA; SOIL;
D O I
10.1016/j.envres.2023.115899
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The primary objective of this study is to synthesize the magnetically separable highly active porous immobilized laccase for the removal of pentachlorophenol (PCP) in an aqueous solution. Magnetic porous cross-linked enzyme aggregates (Mp-CLEAs) of laccase were synthesized using 1% starch solution with 5 mM glutaraldehyde followed by 10 h of cross-linking time with an activity recovery of 90.85 +/- 0.2%. The biocatalytic efficiency of magnetic porous CLEAs (Mp-CLEAs) was 2-fold higher than that of magnetic CLEAs. The synthesized Mp-CLEAs were mechanically stable with enhanced catalytic efficiency, and reusability thus overcoming the mass transfer lim-itations and enzyme loss. At 40 degrees C, the thermal stability of the magnetic porous immobilized laccase was improved, with a 602 min half-life compared to 207 min half-life for the free enzyme. Using 40 U/mL of laccase for the removal of 100 ppm of PCP, M-CLEAs, and Mp-CLEAs removed 60.44% and 65.53% of PCP, respectively. Furthermore, to enhance PCP removal, a laccase-aided system was harnessed by optimizing various surfactants and mediators. Of these, 0.1 mM of rhamnolipid and 2,3 dimethoxy phenol had the highest PCP removal rates of 95.12% and 99.41%, respectively, for Mp-CLEAs. This study demonstrates the efficacy of the laccase-surfactant-mediator system for the removal of PCP from the aqueous solution, which can also be proposed for real-time application.
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页数:9
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共 71 条
  • [1] Ahmed P. M., 2022, Springer Protocols Handbooks, P139, DOI [10.1007/978-1-0716-2006-912, DOI 10.1007/978-1-0716-2006-912]
  • [2] Surfactant efficiency on pentachlorophenol-contaminated wastewater enhanced by Pseudomonas putida AJ 785569
    Ammeri, Rim Werheni
    Hidri, Yassine
    Hassen, Wafa
    Mehri, Ines
    Khlifi, Nesrine
    Hassen, Abdennaceur
    [J]. ARCHIVES OF MICROBIOLOGY, 2021, 203 (08) : 5141 - 5152
  • [3] Surfactant-assisted two phase partitioning bioreactors for laccase-catalyzed degradation of anthracene
    Arca-Ramos, A.
    Eibes, G.
    Moreira, M. T.
    Feijoo, G.
    Lema, J. M.
    [J]. PROCESS BIOCHEMISTRY, 2012, 47 (07) : 1115 - 1121
  • [4] Removal of phenol and bisphenol-A catalyzed by laccase in aqueous solution
    Asadgol, Zahra
    Forootanfar, Hamid
    Rezaei, Shahla
    Mahvi, Amir Hossein
    Faramarzi, Mohammad Ali
    [J]. JOURNAL OF ENVIRONMENTAL HEALTH SCIENCE AND ENGINEERING, 2014, 12
  • [5] Impacts of redox-mediator type on trace organic contaminants degradation by laccase: Degradation efficiency, laccase stability and effluent toxicity
    Ashe, Bradley
    Nguyen, Luong N.
    Hai, Faisal I.
    Lee, Duu-Jong
    van de Merwe, Jason P.
    Leusch, Frederic D. L.
    Price, William E.
    Nghiem, Long D.
    [J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2016, 113 : 169 - 176
  • [6] Characterization and kinetic properties of the purified Trematosphaeria mangrovei laccase enzyme
    Atalla, M. Mabrouk
    Zeinab, H. Kheiralla
    Eman, R. Hamed
    Amani, A. Youssry
    Abeer, A. Abd El Aty
    [J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2013, 20 (04) : 373 - 381
  • [7] Ba S., 2012, Journal of Enzyme Research, V3, P23
  • [8] Utilization of Cross-Linked Laccase Aggregates in a Perfusion Basket Reactor for the Continuous Elimination of Endocrine-Disrupting Chemicals
    Cabana, Hubert
    Jones, J. Peter
    Agathos, Spiros N.
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2009, 102 (06) : 1582 - 1592
  • [9] Immobilization of fungal laccase onto a nonionic surfactant-modified clay material: application to PAH degradation
    Chang, Yi-Tang
    Lee, Jiunn-Fwu
    Liu, Keng-Hua
    Liao, Yi-Fen
    Yang, Vivian
    [J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2016, 23 (05) : 4024 - 4035
  • [10] Sustainable intensification: overcoming land and water constraints on food production
    Chartres, Colin J.
    Noble, Andrew
    [J]. FOOD SECURITY, 2015, 7 (02) : 235 - 245