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Generation and Characterization of ORF55/ORF57-Deleted Recombinant Cyprinid herpesvirus 2 Mutants with Chimeric Capsid Protein Gene of Grouper Nervous Necrosis Virus
被引：1
|作者：
Feng, Zizhao
[1
,2
,3
]
Cheng, Wenjie
[1
,2
,3
]
Ma, Mingyang
[1
,2
,3
]
Yu, Chenwei
[1
,2
,3
]
Zhang, Ye
[1
,2
,3
]
Lu, Liqun
[1
,2
,3
]
Wang, Hao
[1
,2
,3
]
Gui, Lang
[1
,2
,3
]
Xu, Dan
[1
,2
,3
]
Dong, Chuanfu
[4
]
机构：
[1] Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai 201306, Peoples R China
[2] Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
[3] Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai 201306, Peoples R China
[4] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
来源：
关键词：
CyHV-2;
RGNNV;
virulence;
homologous recombination;
viral vector;
INFECTIOUS PANCREATIC NECROSIS;
HEMATOPOIETIC NECROSIS;
CARASSIUS-GIBELIO;
IMMUNE-RESPONSE;
CARP;
IDENTIFICATION;
VACCINATION;
EXPRESSION;
VACCINES;
DELETION;
D O I：
10.3390/vaccines12010043
中图分类号：
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Cyprinid herpesvirus 2 (CyHV-2) is a pathogen that causes significant losses to the global aquaculture industry due to mass mortality in crucian carp and goldfish. This study demonstrates that the ORF55/ORF57 deletion mutants CyHV-2-Delta 55-CP and CyHV-2-Delta 57-CP obtained through homologous recombination replicate effectively within the caudal fin of Carassius auratus gibelio (GiCF) cells and exhibit morphologies similar to the CyHV-2 wild-type strain. Both mutants demonstrated a decrease in virulence, with CyHV-2-Delta 57-CP exhibiting a more significant reduction. This serves as a reference for the subsequent development of recombinant attenuated vaccines against CyHV-2. Additionally, both mutants expressed the inserted RGNNV-CP (capsid protein of Redspotted grouper nervous necrosis virus) fusion protein gene, and inoculation with CyHV-2-Delta 57-CP-infected GiCF cell lysates elicited an antibody response in the grouper. These results indicate that, while ORF55 and ORF57 genes of CyHV-2 are not required for viral replication in vitro, they do play a role in virulence in vivo. Additionally, expression of foreign protein in CyHV-2 suggests that the fully attenuated mutant of CyHV-2 could potentially function as a viral vector for developing subunit vaccines or multivalent recombinant attenuated vaccines.
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页数：18
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