Validation and Application of a Molecular Detection System for Fusarium Wilt of Banana in China

被引:3
|
作者
Yang, Di [1 ]
Du, Chanjuan [1 ]
Zhang, Jin [1 ]
Pan, Lianfu [1 ]
Wei, Shaolong [1 ]
Jiang, Shangbo [1 ]
Li, Chunyu [2 ]
San, Cho Cho [3 ]
Huy, Nguyen Duc [4 ]
Ye, Yunfeng [5 ]
Fu, Gang [1 ]
机构
[1] Guangxi Acad Agr Sci, Plant Protect Res Inst, Key Lab Green Prevent & Control Fruits & Vegetable, Minist Agr & Rural Affairs,Guangxi Key Lab Biol Cr, Nanning 530007, Peoples R China
[2] Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China
[3] Dept Agr Res, Plant Pathol Res Sect, Naypyitaw 15013, Myanmar
[4] Vietnam Natl Univ Agr, Fac Agron, Dept Plant Pathol, Hanoi 100803, Vietnam
[5] Guangxi Acad Agr Sci, Hort Res Inst, Nanning 530007, Peoples R China
关键词
Fusarium oxysporum f. sp. cubense; molecular detection; primer evaluation; F-SP CUBENSE; TROPICAL RACE 4; SP; CUBENSE; IDENTIFICATION; DIAGNOSIS; FUNGUS;
D O I
10.1094/PDIS-04-23-0618-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Fusarium wilt of banana is a devastating disease caused by Fusarium oxysporum f. sp. cubense (Foc). It has restricted the development of the banana industry worldwide and is particularly serious in China because of the large planting areas and special planting patterns. However, there is no rapid and accurate approach to detect the Foc strains that specifically occur in China because of the rich genetic diversity observed in this pathosystem. In this study, we evaluated the performance of 10 previously published PCR primer pairs on 103 representative Foc strains in China and neighboring countries and screened out a set of primers (Foc-specific primer pair SIX9-Foc-F/R, Foc R1-specific primer pair SIX6b-210-F/R, Foc R4-specific primer pair Foc-1/2, and Foc TR4-specific primer pair W2987F/R) suitable for the detection of Foc strains in China and the surrounding Southeast Asian countries. Moreover, we developed a molecular detection system to accurately identify the different physiological races of Foc. The findings of this study provide technical support for preventing and controlling the spread of Fusarium wilt of banana in the field in China.
引用
收藏
页码:3687 / 3692
页数:6
相关论文
共 50 条
  • [41] Distribution and diversity of Fusarium oxysporum f.sp. cubense TR4 causing banana wilt in Sub-tropics of India and comparative analysis of TR4 specific molecular detection methods
    Kumari, Nidhi
    Damodaran, Thukkaram
    Ahmad, Israr
    Rajan, Shailendra
    Shukla, P. K.
    Manoharan, Muthukumar
    Kushwaha, Amar Kant
    Singh, Haripal
    Gopal, Ram
    Kumari, Sangeeta
    Yadav, Kavita
    Bora, Popy
    Jha, Sunil Kumar
    JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2023, 32 (03) : 570 - 586
  • [42] Histological and Gene Expression Analyses in Banana Reveals the Pathogenic Differences between Races 1 and 4 of Banana Fusarium Wilt Pathogen
    Dong, Honghong
    Fan, Huiyun
    Lei, Zhaoxi
    Wu, Chao
    Zhou, Dengbo
    Li, Huaping
    PHYTOPATHOLOGY, 2019, 109 (06) : 1029 - 1042
  • [43] Preference of Cosmopolites sordidus for fusarium wilt-diseased banana plants
    Guillen Sanchez, Cesar
    de Lapeyre de Bellaire, Luc
    Sandoval Fernandez, Jorge Arturo
    Tixier, Philippe
    JOURNAL OF APPLIED ENTOMOLOGY, 2023, 147 (02) : 140 - 146
  • [44] Nitrogen fertilizer rate but not form affects the severity of Fusarium wilt in banana
    Orr, Ryan
    Dennis, Paul G.
    Wong, Yide
    Browne, Daniel J.
    Cooper, Martha
    Birt, Henry W. G.
    Lapis-Gaza, Hazel R.
    Pattison, Anthony B.
    Nelson, Paul N.
    FRONTIERS IN PLANT SCIENCE, 2022, 13
  • [45] Fusarium Wilt of Banana: Current Knowledge on Epidemiology and Research Needs Toward Sustainable Disease Management
    Dita, Miguel
    Barquero, Marcia
    Heck, Daniel
    Mizubuti, Eduardo S. G.
    Staver, Charles P.
    FRONTIERS IN PLANT SCIENCE, 2018, 9
  • [46] Banana Fusarium Wilt Disease Detection by Supervised and Unsupervised Methods from UAV-Based Multispectral Imagery
    Zhang, Shimin
    Li, Xiuhua
    Ba, Yuxuan
    Lyu, Xuegang
    Zhang, Muqing
    Li, Minzan
    REMOTE SENSING, 2022, 14 (05)
  • [47] Continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana Fusarium wilt suppression
    Fu, Lin
    Ruan, Yunze
    Tao, Chengyuan
    Li, Rong
    Shen, Qirong
    SCIENTIFIC REPORTS, 2016, 6
  • [48] First specific detection and validation of tomato wilt caused by Fusarium brachygibbosum using a PCR assay
    Deng, Siyi
    Liu, Quanke
    Chang, Wei
    Liu, Jun
    Wang, Hua
    PEERJ, 2023, 11
  • [49] Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control
    Rajakumar, Priya
    Baharum, Nadiya Akmal
    Lutfi, Afiqah Insyirah
    Sadali, Najiah Mohd
    Mispan, Muhamad Shakirin
    Kuang, Lim Lian
    Ling, Yap Seong
    Khalid, Norzulaani
    Rejab, Nur Ardiyana
    PLANT PATHOLOGY JOURNAL, 2024, 40 (05) : 463 - 474
  • [50] Agronomic characterization and identification of banana genotypes resistant to Fusarium wilt race 1
    Goncalves, Zalmar Santana
    Haddad, Fernando
    de Oliveira Amorim, Vanusia Batista
    Ferreira, Claudia Fortes
    Santos de Oliveira, Saulo Alves
    Amorim, Edson Perito
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2019, 155 (04) : 1093 - 1103