Gene expression analysis during the conversion from a viable but nonculturable to culturable state in Vibrio cholerae

被引:4
作者
Ayibieke, Alafate [1 ]
Nishiyama, Ayae [1 ,3 ]
Senoh, Mitsutoshi [2 ]
Hamabata, Takashi [1 ]
机构
[1] Natl Ctr Global Hlth & Med, Res Inst, 1-21-1 Toyama,Shinjuku Ku, Tokyo 1628655, Japan
[2] Natl Inst Infect Dis, Dept Bacteriol 2, 4-7-1 Gakuen, Tokyo 2080011, Japan
[3] Natl Inst Hlth & Nutr, 1-23-1 Toyama,Shinjuku Ku, Tokyo 1628636, Japan
关键词
Viable but non-culturable cell; Vibrio cholerae; Catalase-induced conversion; Gene expression; RNA microarray; ESCHERICHIA-COLI; MECHANOSENSITIVE CHANNELS; STATIONARY-PHASE; SURVIVAL; COCULTURE; VIABILITY; VIRULENCE; ISCR; O1;
D O I
10.1016/j.gene.2023.147289
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We previously reported that Vibrio cholerae in a viable but non-culturable (VBNC) state can be converted to a culturable state by treatment with catalase. This finding enabled us to develop an assay system to observe the time course of the conversion from VBNC to culturable in V. cholerae. VBNC cells began to convert to culturable cells as early as 2 h after catalase supplementation. Gene expression in VBNC cells during catalase treatment was analyzed using RNA microarray. Many ribosomal DNA genes were stimulated 6 h post catalase exposure, sug-gesting that the conversion-driving signal started prior to 6 h. Focusing on the period prior to cell proliferation, we found that 16 genes might be involved in the conversion mechanism in V. cholerae, and they showed enhanced expression at 2 h and 4 h after catalase addition. These upregulated genes included phage shock proteins (pspA, B, and C), alternative sigma factor (rpoE) and its negative regulator (rseA), cobW C terminal domain-containing protein, damage-inducible helicase (dinG), cholerae toxin secretion protein epsM, HTH-type transcription regulator (iscR), mechanosensitive ion channel family protein, anthranilate synthase component I, fructose-specific IIBC component, molybdenum import ATP-binding protein (modC), LysE family translocator, putative organic hydroperoxide resistance protein, and a hypothetical protein. This study identified genes involved in the catalase-induced conversion of V. cholerae VBNC cells to a culturable state and provided valuable insights into the mechanisms involved in the conversion process.
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页数:9
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共 34 条
[1]   New envelope stress factors involved in σE activation and conditional lethality of rpoE mutations in Salmonella enterica [J].
Amar, Agustina ;
Pezzoni, Magdalena ;
Pizarro, Ramon A. ;
Costa, Cristina S. .
MICROBIOLOGY-SGM, 2018, 164 (10) :1293-1307
[2]   Gene expression profile of Vibrio cholerae in the cold stress-induced viable but non-culturable state [J].
Asakura, Hiroshi ;
Ishiwa, Akiko ;
Arakawa, Eiji ;
Makino, Sou-ichi ;
Okada, Yumiko ;
Yamamoto, Shigeki ;
Igimi, Shizunobu .
ENVIRONMENTAL MICROBIOLOGY, 2007, 9 (04) :869-879
[3]   Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples [J].
Bari, S. M. Nayeemul ;
Roky, M. Kamruzzaman ;
Mohiuddin, M. ;
Kamruzzaman, M. ;
Mekalanos, John J. ;
Faruque, Shah M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (24) :9926-9931
[4]   Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae [J].
Brenzinger, Susanne ;
van der Aart, Lizah T. ;
van Wezel, Gilles P. ;
Lacroix, Jean-Marie ;
Glatter, Timo ;
Briegel, Ariane .
FRONTIERS IN MICROBIOLOGY, 2019, 10
[5]   VIABLE BUT NON-CULTURABLE VIBRIO-CHOLERAE AND RELATED PATHOGENS IN THE ENVIRONMENT - IMPLICATIONS FOR RELEASE OF GENETICALLY ENGINEERED MICROORGANISMS [J].
COLWELL, RR ;
BRAYTON, PR ;
GRIMES, DJ ;
ROSZAK, DB ;
HUQ, SA ;
PALMER, LM .
BIO-TECHNOLOGY, 1985, 3 (09) :817-820
[6]   Viable but non culturable Vibrio cholerae 01 revert to a cultivable state in the human intestine [J].
Colwell, RR ;
Brayton, P ;
Herrington, D ;
Tall, B ;
Huq, A ;
Levine, MM .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (01) :28-31
[7]   ppGpp and DksA likely regulate the activity of the extracytoplasmic stress factor σE in Escherichia coli by both direct and indirect mechanisms [J].
Costanzo, Alessandra ;
Nicoloff, Herve ;
Barchinger, Sarah E. ;
Banta, Amy B. ;
Gourse, Richard L. ;
Ades, Sarah E. .
MOLECULAR MICROBIOLOGY, 2008, 67 (03) :619-632
[8]   Characterization of the Vibrio cholerae Phage Shock Protein Response [J].
DeAngelis, Cara M. ;
Nag, Dhrubajyoti ;
Withey, Jeffrey H. ;
Matson, Jyl S. .
JOURNAL OF BACTERIOLOGY, 2019, 201 (14)
[9]   Comparative proteomic analysis to characterize temperature-induced viable but non-culturable and resuscitation states in Vibrio cholerae [J].
Debnath, Anusuya ;
Mizuno, Tamaki ;
Miyoshi, Shin-ichi .
MICROBIOLOGY-SGM, 2019, 165 (07) :737-746
[10]   Bacterial Approaches for Assembling Iron-Sulfur Proteins [J].
Esquilin-Lebron, Karla ;
Dubrac, Sarah ;
Barras, Frederic ;
Boyd, Jeffrey M. .
MBIO, 2021, 12 (06)