SARS-CoV-2 ORF3a positively regulates NF-?B activity by enhancing IKK?-NEMO interaction

被引：11

作者：

Nie, Ying ^{[1
]}

Mou, Lumin ^{[1
,2
]}

Long, Qizhou ^{[1
]}

Deng, Dongqing ^{[1
]}

Hu, Rongying ^{[3
]}

Cheng, Jinzhi ^{[1
]}

Wu, Jiahong ^{[1
,2
]}

机构：

[1] Guizhou Med Univ, Coll Basic Med Sci, Dept Parasitol, Prov Key Lab Modern Pathogen Biol, Guiyang 550025, Peoples R China

[2] Guizhou Med Univ, Sch Publ Hlth, Key Lab Environm Pollut Monitoring & Dis Control, Minist Educ, Guiyang 550025, Peoples R China

[3] Guizhou Med Univ, Affiliated Hosp, Guiyang 550004, Peoples R China

来源：

VIRUS RESEARCH | 2023年 / 328卷

基金：

中国国家自然科学基金;

关键词：

SARS-CoV-2; ORF3a; Inflammation; NF-?B; IKK; NEMO; KAPPA-B; NLRP3; INFLAMMASOME; INHIBITION; APOPTOSIS; CANCER; IMMUNITY; PROTEIN; BETA; LINE;

D O I：

10.1016/j.virusres.2023.199086

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Coronavirus disease 2019 (COVID-19) is a global pandemic caused by SARS-CoV-2 infection. Patients with severe COVID-19 exhibit robust induction of proinflammatory cytokines, which are closely associated with the devel-opment of acute respiratory distress syndrome. However, the underlying mechanisms of the NF-kappa B activation mediated by SARS-CoV-2 infection remain poorly understood. Here, we screened SARS-CoV-2 genes and found that ORF3a induces proinflammatory cytokines by activating the NF-kappa B pathway. Moreover, we found that ORF3a interacts with IKK beta and NEMO and enhances the interaction of IKK beta-NEMO, thereby positively regulating NF-kappa B activity. Together, these results suggest ORF3a may play pivotal roles in the pathogenesis of SARS-CoV-2 and provide novel insights into the interaction between host immune responses and SARS-CoV-2 infection.

引用

页数：9

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