Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects

被引:4
作者
Tseng, Kuo-Fang [1 ]
Shiu, Shiau-Ting [1 ,2 ]
Hung, Chia-Yi [1 ,3 ]
Chan, Ya-Hui [4 ]
Chee, Tze-Jian [1 ]
Huang, Pai-Chun [1 ]
Lai, Pin-Chuang [5 ]
Feng, Sheng-Wei [1 ,3 ,6 ]
机构
[1] Taipei Med Univ, Coll Oral Med, Sch Dent, Taipei City 10301, Taiwan
[2] Taipei Med Univ, Shuang Ho Hosp, Dept Dent, New Taipei City 35041, Taiwan
[3] Natl Def Med Ctr, Grad Inst Dent Sci, Sch Dent, Taipei City 114201, Taiwan
[4] Taipei Med Univ, Coll Oral Med, Sch Oral Hyg, Taipei City 110301, Taiwan
[5] Univ Missouri Kansas City, Sch Dent, Dept Periodont, Kansas City, MO 64108 USA
[6] Taipei Med Univ Hosp, Dept Dent, Div Prosthodont, Taipei City 11031, Taiwan
关键词
implant stability; osseointegration; peri-implant defect; bone graft materials; mesenchymal stem cells; ALGAE-DERIVED HYDROXYAPATITE; REGENERATION; SUBSTITUTES; PHOSPHATE; TIBIA;
D O I
10.3390/ijms25020862
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many studies have been exploring the use of bone graft materials (BGMs) and mesenchymal stem cells in bone defect reconstruction. However, the regeneration potential of Algipore (highly purified hydroxyapatite) and Biphasic (hydroxyapatite/beta-tricalcium phosphate) BGMs combined with bone marrow-derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we evaluated their osseointegration capacities in reconstructing peri-implant bone defects. The cellular characteristics of BMSCs and the material properties of Algipore and Biphasic were assessed in vitro. Four experimental groups-Algipore, Biphasic, Algipore+BMSCs, and Biphasic+BMSCs-were designed in a rabbit tibia peri-implant defect model. Implant stability parameters were measured. After 4 and 8 weeks of healing, all samples were evaluated using micro-CT, histological, and histomorphometric analysis. In the energy-dispersive X-ray spectroscopy experiment, the Ca/P ratio was higher for Algipore (1.67) than for Biphasic (1.44). The ISQ values continuously increased, and the PTV values gradually decreased for all groups during the healing period. Both Algipore and Biphasic BGM promoted new bone regeneration. Higher implant stability and bone volume density were observed when Algipore and Biphasic BGMs were combined with BMSCs. Biphasic BGM exhibited a faster degradation rate than Algipore BGM. Notably, after eight weeks of healing, Algipore with BSMCs showed more bone-implant contact than Biphasic alone (p < 0.05). Both Algipore and Biphasic are efficient in reconstructing peri-implant bone defects. In addition, Algipore BGM incorporation with BSMCs displayed the best performance in enhancing implant stability and osseointegration potential.
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页数:14
相关论文
共 42 条
[1]   The Biomaterial-Induced Cellular Reaction Allows a Novel Classification System Regardless of the Biomaterials Origin [J].
Al-Maawi, Sarah ;
Rutkowski, James L. ;
Sader, Robert ;
Kirkpatrick, C. James ;
Ghanaati, Shahram .
JOURNAL OF ORAL IMPLANTOLOGY, 2020, 46 (03) :190-205
[2]   Bone Marrow Mesenchymal Stromal Cells (BMMSCs) Augment Osteointegration of Dental Implants in Type 1 Diabetic Rabbits: An X-Ray Micro-Computed Tomographic Evaluation [J].
Alqahtani, Nabeeh Abdullah ;
Chandramoorthy, Harish C. ;
Shaik, Sharaz ;
Syed, Jamaluddin ;
Chowdhary, Ramesh ;
Antony, Leoney .
MEDICINA-LITHUANIA, 2020, 56 (04)
[3]   Evaluation of Peri-Implant Bone Grafting Around Surface-Porous Dental Implants: An In Vivo Study in a Goat Model [J].
Alshehri, Fahad ;
Alshehri, Mohammed ;
Sumague, Terrence ;
Niazy, Abdurahman ;
Jansen, John ;
van den Beucken, Jeroen ;
Alghamdi, Hamdan .
MATERIALS, 2019, 12 (21)
[4]   Melatonin enhances osteogenic differentiation of dental pulp mesenchymal stem cells by regulating MAPK pathways and promotes the efficiency of bone regeneration in calvarial bone defects [J].
Chan, Ya-Hui ;
Ho, Kuo-Ning ;
Lee, Yu-Chieh ;
Chou, Meng-Jung ;
Lew, Wei-Zhen ;
Huang, Haw-Ming ;
Lai, Pin-Chuang ;
Feng, Sheng-Wei .
STEM CELL RESEARCH & THERAPY, 2022, 13 (01)
[5]   An evaluation of the biocompatibility and osseointegration of novel glass fiber reinforced composite implants: In vitro and in vivo studies [J].
Chan, Ya-Hui ;
Lew, Wei-Zhen ;
Lu, Emily ;
Loretz, Thomas ;
Lu, Luke ;
Lin, Che-Tong ;
Feng, Sheng-Wei .
DENTAL MATERIALS, 2018, 34 (03) :470-485
[6]   Characteristics of contact and distance osteogenesis around modified implant surfaces in rabbit tibiae [J].
Choi, Jung-Yoo ;
Sim, Jae-Hyuk ;
Yeo, In-Sung Luke .
JOURNAL OF PERIODONTAL AND IMPLANT SCIENCE, 2017, 47 (03) :182-192
[7]   A systematic review on the effect of inorganic surface coatings in large animal models and meta-analysis on tricalcium phosphate and hydroxyapatite on periimplant bone formation [J].
Damerau, Jeanne-Marie ;
Bierbaum, Susanne ;
Wiedemeier, Daniel ;
Korn, Paula ;
Smeets, Ralf ;
Jenny, Gregor ;
Nadalini, Johanna ;
Stadlinger, Bernd .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2022, 110 (01) :157-175
[8]  
Davies JE, 1998, INT J PROSTHODONT, V11, P391
[9]   Bone Regeneration of Canine Peri-implant Defects Using Cell Sheets of Adipose-Derived Mesenchymal Stem Cells and Platelet-Rich Fibrin Membranes [J].
Ding, Lidan ;
Tang, Shijun ;
Liang, Panpan ;
Wang, Chao ;
Zhou, Peng-fei ;
Zheng, Leilei .
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2019, 77 (03) :499-514
[10]   Evaluation of bone substitutes for treatment of peri-implant bone defects: biomechanical, histological, and immunohistochemical analyses in the rabbit tibia [J].
dos Santos, Pamela Leticia ;
de Molon, Rafael Scaf ;
Queiroz, Thallita Pereira ;
Okamoto, Roberta ;
de Souza Faloni, Ana Paula ;
Gulinelli, Jessica Lemos ;
Luvizuto, Eloa Rodrigues ;
Garcia Junior, Idelmo Rangel .
JOURNAL OF PERIODONTAL AND IMPLANT SCIENCE, 2016, 46 (03) :176-196