Down-Regulation of miRNA-1303 Promotes the Angiogenesis of HUVECs via Targeting THSD7A

被引：0

作者：

Xiang, Guoliang ^{[1
,3
]}

Zhao, Yanan ^{[1
,3
]}

Jin, Di ^{[1
,3
]}

Fang, Yanbo ^{[1
,3
]}

Li, Zhiyi ^{[1
,3
]}

He, Xiaofeng ^{[1
,3
]}

Zhai, Yifei ^{[1
,3
]}

Teng, Junfang ^{[1
,2
,3
]}

Deng, Wenjing ^{[1
,3
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Neurol Intens Care Unit, Zhengzhou 450052, Henan, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Neurol, Zhengzhou 450052, Henan, Peoples R China

[3] Zhengzhou Univ, Affiliated Hosp 1, Henan Prov Neurol Dis Med Ctr, Zhengzhou 450052, Henan, Peoples R China

来源：

MOLECULAR BIOTECHNOLOGY | 2024年 / 66卷 / 10期

关键词：

Acute ischemic stroke; Angiogenesis; HUVECs; miR-1303; THSD7A; DOMAIN-CONTAINING; 7A; CEREBRAL-ISCHEMIA; NEUROPLASTICITY; TRANSLATION; MICRORNAS; MECHANISM; STROKE;

D O I：

10.1007/s12033-023-00906-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiogenesis promotes neurological recovery after acute ischemic stroke (AIS), and microRNAs play crucial roles in cerebral angiogenesis. This study found that Homo sapiens-microRNA-1303(miR-1303) was reduced in blood specimens of AIS patients and human umbilical vein endothelial cells after suffering from oxygen-glucose deprivation/reperfusion. The experiment detected the effect of miR-1303 on angiogenesis by wound healing assay, tube formation assay, and transwell assay. Down-regulation of miRNA-1303 promotes angiogenesis in vitro experiments, while miR-1303 over-expression reverses this effect. Based on bioinformatics analyses and dual-luciferase reporter assay, the thrombospondin type 1 domain containing 7A (THSD7A) was investigated and further validated as the downstream gene of miR-1303. Furthermore, the knockdown of miR-1303 decreased the protein translation and mRNA transcript levels of THSD7A. Our results reveal a novel miR-1303/THSD7A pathway for angiogenesis and further imply that miR-1303 can be a promising biomarker and therapeutic target for AIS.

引用

页码：2897 / 2908

页数：12