Down-Regulation of miRNA-1303 Promotes the Angiogenesis of HUVECs via Targeting THSD7A

被引：0
作者：
Xiang, Guoliang [1 ,3 ]
Zhao, Yanan [1 ,3 ]
Jin, Di [1 ,3 ]
Fang, Yanbo [1 ,3 ]
Li, Zhiyi [1 ,3 ]
He, Xiaofeng [1 ,3 ]
Zhai, Yifei [1 ,3 ]
Teng, Junfang [1 ,2 ,3 ]
Deng, Wenjing [1 ,3 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Neurol Intens Care Unit, Zhengzhou 450052, Henan, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Neurol, Zhengzhou 450052, Henan, Peoples R China
[3] Zhengzhou Univ, Affiliated Hosp 1, Henan Prov Neurol Dis Med Ctr, Zhengzhou 450052, Henan, Peoples R China
来源：
MOLECULAR BIOTECHNOLOGY | 2024年 / 66卷 / 10期
关键词：
Acute ischemic stroke; Angiogenesis; HUVECs; miR-1303; THSD7A; DOMAIN-CONTAINING; 7A; CEREBRAL-ISCHEMIA; NEUROPLASTICITY; TRANSLATION; MICRORNAS; MECHANISM; STROKE;
D O I：
10.1007/s12033-023-00906-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Angiogenesis promotes neurological recovery after acute ischemic stroke (AIS), and microRNAs play crucial roles in cerebral angiogenesis. This study found that Homo sapiens-microRNA-1303(miR-1303) was reduced in blood specimens of AIS patients and human umbilical vein endothelial cells after suffering from oxygen-glucose deprivation/reperfusion. The experiment detected the effect of miR-1303 on angiogenesis by wound healing assay, tube formation assay, and transwell assay. Down-regulation of miRNA-1303 promotes angiogenesis in vitro experiments, while miR-1303 over-expression reverses this effect. Based on bioinformatics analyses and dual-luciferase reporter assay, the thrombospondin type 1 domain containing 7A (THSD7A) was investigated and further validated as the downstream gene of miR-1303. Furthermore, the knockdown of miR-1303 decreased the protein translation and mRNA transcript levels of THSD7A. Our results reveal a novel miR-1303/THSD7A pathway for angiogenesis and further imply that miR-1303 can be a promising biomarker and therapeutic target for AIS.
引用
收藏
页码：2897 / 2908
页数：12
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