Protective effect of ginsenoside Rg1 on 661W cells exposed to oxygen-glucose deprivation/reperfusion via keap1/nrf2 pathway

被引:0
|
作者
Zhou, Ming [1 ]
Ma, Xin-Qi [1 ]
Xie, Yi-Yu [1 ]
Zhou, Jia-Bei [1 ]
Kuang, Xie-Lan [1 ]
Shen, Huang-Xuan [1 ]
Long, Chong-De [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangdong Prov Key Lab Ophthalmol & Visual Sci, Guangzhou 510060, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, Guangdong Prov Key Lab Ophthalmol & Visual Sci, Guangzhou 510060, Peoples R China
关键词
oxygen-glucose deprivation; reoxygenation; ginsenoside Rg1; oxidative stress; phosphorylated nrf2; ISCHEMIA; INJURY;
D O I
10.18240/ijo.2023.07.04
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
? AIM: To construct an in vitro model of oxygen-glucose deprivation/reperfusion (OGD/R) induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion (I/R) injury in 661W cells and the protective effect of ginsenoside Rg1. ? METHODS: The 661W cells were treated with different concentrations of Na2S2O4 to establish OGD/R model in vitro. Apoptosis, intracellular reactive oxygen species (ROS) levels and superoxide dismutase (SOD) levels were measured at different time points during the reperfusion injury process. The injury model was pretreated with graded concentrations of ginsenoside Rg1. Real-time polymerase chain reaction (PCR) was used to measure the expression levels of cytochrome C (cyt C)/B-cell lymphoma-2 (Bcl2)/ Bcl2 associated protein X (Bax), heme oxygenase-1 (HO-1), caspase9, nuclear factor erythroid 2-related factor 2 (nrf2), kelch-like ECH-associated protein 1 (keap1) and other genes. Western blot was used to detect the expression of nrf2, phosphorylated nrf2 (pnrf2) and keap1 protein levels. ? RESULTS: Compared to the untreated group, the cell activity of 661W cells treated with Na2S2O4 for 6 and 8h decreased (P<0.01). Additionally, the ROS content increased and SOD levels decreased significantly (P<0.01). In contrast, treatment with ginsenoside Rg1 reversed the cell viability and SOD levels in comparison to the Na2S2O4 treated group (P<0.01). Moreover, Rg1 reduced the levels of caspase3, caspase9, and cytC, while increasing the Bcl2/ Bax level. These differences were all statistically significant (P<0.05). Western blot analysis showed no significant difference in the protein expression levels of keap1 and nrf2 with Rg1 treatment, however, Rg1 significantly increased the ratio of pnrf2/nrf2 protein expression compared to the Na2S2O4 treated group (P<0.001). ? CONCLUSION: The OGD/R process is induced in 661W cells using Na2S2O4. Rg1 inhibits OGD/R-induced oxidative damage and alleviates the extent of apoptosis in 661W cells through the keap1/nrf2 pathway. These results suggest a potential protective effect of Rg1 against retinal I/R injury.
引用
收藏
页码:1026 / 1033
页数:8
相关论文
共 50 条
  • [1] Protective effect of ginsenoside Rg1 on 661W cells exposed to oxygen-glucose deprivation/reperfusion via keap1/nrf2 pathway
    Ming Zhou
    Xin-Qi Ma
    Yi-Yu Xie
    Jia-Bei Zhou
    Xie-Lan Kuang
    Huang-Xuan Shen
    Chong-De Long
    International Journal of Ophthalmology, 2023, (07) : 1026 - 1033
  • [2] Neuroprotective effects of ginsenoside Rg1 on oxygen-glucose deprivation reperfusion in PC12 cells
    Huang, Sheng-Li
    He, Xi-Jing
    Li, Zong-Fang
    Lin, Lei
    Cheng, Bin
    PHARMAZIE, 2014, 69 (03): : 208 - 211
  • [3] Ginsenoside Rg1 regulating inflammatory response and bone-remodeling through Keap1/Nrf2 signaling pathway in rats with periodontitis
    Zhou, Yang
    Zhang, Yunan
    Wang, Li
    Liu, Youbo
    Wang, Zhongke
    Guo, Ling
    SCIENTIFIC REPORTS, 2025, 15 (01):
  • [4] Neuroprotective effects of ginsenoside Rg1 against oxygen-glucose deprivation in cultured hippocampal neurons
    He, Qing
    Sun, Jianguo
    Wang, Qin
    Wang, Wei
    He, Bin
    JOURNAL OF THE CHINESE MEDICAL ASSOCIATION, 2014, 77 (03) : 142 - 149
  • [5] Ginsenoside Rg3 attenuates myocardial ischemia/reperfusion-induced ferroptosis via the keap1/Nrf2/GPX4 signaling pathway
    Zhong, Guofu
    Chen, Junteng
    Li, Yangtao
    Han, Yue
    Wang, Maosheng
    Nie, Qinqi
    Xu, Mujuan
    Zhu, Qinghua
    Chang, Xiao
    Wang, Ling
    BMC COMPLEMENTARY MEDICINE AND THERAPIES, 2024, 24 (01)
  • [6] Protective mechanism against oxidative stress by Keap1/Nrf2 pathway
    Itoh, K
    SEIKAGAKU, 2006, 78 (02): : 79 - 92
  • [7] Sevoflurane alleviates oxygen-glucose deprivation/reoxygenation-induced damage in HT22 cells by activating the Keap1/Nrf2/ARE pathway to inhibit oxidative stress
    Yan, Ke
    Li, Lei
    Ye, Siqi
    Xu, Qiao
    Ding, Li
    INTERNATIONAL JOURNAL OF NEUROSCIENCE, 2025, 135 (01) : 110 - 117
  • [8] Effect and Mechanism of Ginsenoside Rg1 on Synaptic Plasticity of Oxygen-Glucose Deprivation/Reoxygenation-Induced Neuronal Injury
    Yan, Chen
    Ma, Yan
    Li, Lin
    Zhang, Meng
    Zhan, Junhua
    Fan, Xiang
    PHARMACOGNOSY MAGAZINE, 2020, 16 (71) : 630 - 636
  • [9] Protective effects of ginsenoside Rg1 against oxygen-glucose-deprivation-induced apoptosis in neural stem cells
    Li, Yiwen
    Suo, Lin
    Liu, Yang
    Li, Huiyun
    Xue, Wenxin
    JOURNAL OF THE NEUROLOGICAL SCIENCES, 2017, 373 : 107 - 112
  • [10] Sestrin1 exerts a cytoprotective role against oxygen-glucose deprivation/reoxygenation-induced neuronal injury by potentiating Nrf2 activation via the modulation of Keap1
    Yang, Fang
    Chen, Ruping
    BRAIN RESEARCH, 2021, 1750