Dual-function C2H2-type zinc-finger transcription factor GmZFP7 contributes to isoflavone accumulation in soybean

被引：38

作者：

Feng, Yue ^{[1
,2
]}

Zhang, Shengrui ^{[1
]}

Li, Jing ^{[1
]}

Pei, Ruili ^{[1
]}

Tian, Ling ^{[1
]}

Qi, Jie ^{[1
]}

Azam, Muhammad ^{[1
]}

Agyenim-Boateng, Kwadwo Gyapong ^{[1
]}

Shaibu, Abdulwahab S. ^{[1
]}

Liu, Yitian ^{[1
]}

Zhu, Zuofeng ^{[2
]}

Li, Bin ^{[1
]}

Sun, Junming ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Res Ctr Crop Mol Breeding, MARA Key Lab Soybean Biol Beijing, 12 Zhongguancun South St, Beijing 100081, Peoples R China

[2] China Agr Univ, State Key Lab Plant Physiol & Biochem, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

来源：

NEW PHYTOLOGIST | 2023年 / 237卷 / 05期

基金：

中国国家自然科学基金;

关键词：

dual function; GmZFP7; haplotype; isoflavones; soybean [Glycine max (L; ) Merrill; GENE-EXPRESSION; BIOSYNTHESIS; IDENTIFICATION; PROTEIN; SOY; REGULATOR; GENISTEIN; ACTIVATOR; GMMYB176; LEGUME;

D O I：

10.1111/nph.18610

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Isoflavones are a class of secondary metabolites produced by legumes and play important roles in human health and plant stress tolerance. The C2H2 zinc-finger transcription factor (TF) functions in plant stress tolerance, but little is known about its function in isoflavone regulation in soybean (Glycine max). Here, we report a C2H2 zinc-finger TF gene, GmZFP7, which regulates isoflavone accumulation in soybean. Overexpressing GmZFP7 increased the isoflavone concentration in both transgenic hairy roots and plants. By contrast, silencing GmZFP7 expression significantly reduced isoflavone levels. Metabolomic and qRT-PCR analysis revealed that GmZFP7 can increase the flux of the phenylpropanoid pathway. Furthermore, dual-luciferase and electrophoretic mobility shift assays showed that GmZFP7 regulates isoflavone accumulation by influencing the expression of Isoflavone synthase 2 (GmIFS2) and Flavanone 3 beta-hydroxylase 1 (GmF3H1). In this study, we demonstrate that GmZFP7 contributes to isoflavone accumulation by regulating the expression of the gateway enzymes (GmIFS2 and GmF3H1) of competing phenylpropanoid pathway branches to direct the metabolic flux into isoflavone. A haplotype analysis indicated that important natural variations were present in GmZFP7 promoters, with P-Hap1 and P-Hap3 being the elite haplotypes. Our findings provide insight into how GmZFP7 regulates the phenylpropanoid pathway and enhances soybean isoflavone content.

引用

页码：1794 / 1809

页数：16

共 57 条

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