Protective Effect of Lonicera japonica on PM2.5-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway

被引：16

作者：

Lee, Hyo Lim ^{[1
]}

Kim, Jong Min ^{[1
]}

Go, Min Ji ^{[1
]}

Kim, Tae Yoon ^{[1
]}

Joo, Seung Gyum ^{[1
]}

Kim, Ju Hui ^{[1
]}

Lee, Han Su ^{[1
]}

Kim, Hyun-Jin ^{[1
]}

Heo, Ho Jin ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Inst Agr & Life Sci, Div Appl Life Sci BK21, Jinju 52828, South Korea

来源：

ANTIOXIDANTS | 2023年 / 12卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Lonicera japonica; PM2; 5; pulmonary disease; inflammation; fibrosis; PARTICULATE MATTER; AIR-POLLUTION; AMBIENT PM2.5; EXPOSURE; INFLAMMATION; DYSFUNCTION; EXTRACT; DISEASE;

D O I：

10.3390/antiox12040968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to assess the protective effect of an extract of Lonicera japonica against particulate-matter (PM)(2.5)-induced pulmonary inflammation and fibrosis. The compounds with physiological activity were identified as shanzhiside, secologanoside, loganic acid, chlorogenic acid, secologanic acid, secoxyloganin, quercetin pentoside, and dicaffeoyl quinic acids (DCQA), including 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and 1,4-DCQA using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MSE). The extract of Lonicera japonica reduced cell death, reactive oxygen species (ROS) production, and inflammation in A549 cells. The extract of Lonicera japonica decreased serum T cells, including CD4(+) T cells, CD8(+) T cells, and total T helper 2 (Th2) cells, and immunoglobulins, including immunoglobulin G (IgG) and immunoglobulin E (IgE), in PM2.5-induced BALB/c mice. The extract of Lonicera japonica protected the pulmonary antioxidant system by regulating superoxide dismutase (SOD) activity, reduced glutathione (GSH) contents, and malondialdehyde (MDA) levels. In addition, it ameliorated mitochondrial function by regulating the production of ROS, mitochondrial membrane potential (MMP), and ATP contents. Moreover, the extract of Lonicera japonica exhibited a protective activity of apoptosis, fibrosis, and matrix metalloproteinases (MMPs) via TGF-beta and NF-kappa B signaling pathways in lung tissues. This study suggests that the extract of Lonicera japonica might be a potential material to improve PM2.5-induced pulmonary inflammation, apoptosis, and fibrosis.

引用

页数：21