Relationship between circulating senescence-associated secretory phenotype levels and severity of type 2 diabetes-associated periodontitis: A cross-sectional study

被引:4
作者
Song, Xiuxiu [1 ,2 ]
Yue, Ziqi [1 ,2 ]
Fan, Linli [2 ]
Zou, Haonan [1 ,2 ]
Zhao, Pengfei [1 ,2 ,3 ]
Nie, Lulingxiao [1 ,2 ]
Zhu, Kangjian [1 ,2 ]
Jiang, Jingjing [4 ]
Lv, Qingguo [5 ]
Wang, Qi [1 ,2 ]
机构
[1] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China
[2] Sichuan Univ, Dept Prosthodont, West China Hosp Stomatol, Chengdu, Peoples R China
[3] Univ Hong Kong, Fac Dent, Hong Kong, Peoples R China
[4] Sichuan Univ, Dept Clin Lab, West China Hosp Stomatol, Chengdu, Peoples R China
[5] Sichuan Univ, West China Hosp, Dept Endocrinol & Metab, Chengdu, Peoples R China
基金
中国国家自然科学基金;
关键词
periodontitis; senescence-associated secretory phenotype; type; 2; diabetes; CELLULAR SENESCENCE; 2-WAY ANALYSIS; MECHANISMS; INFLAMMATION; IMMUNITY; RECEPTOR; IMPACT; CELLS;
D O I
10.1002/JPER.22-0445
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
BackgroundSenescence-associated secretory phenotype (SASP) has recently been found to drive comorbid diabetes and periodontitis by inducing a chronic, low-degree inflammatory state. Here, we sought to explore the relationship between circulating SASP and the severity of type 2 diabetes-associated periodontitis (DP). MethodsEighty patients (middle-aged periodontitis, M-P group; aged periodontitis, A-P group; M-DP group; and A-DP group; n = 20) provided gingival epithelium, serum, and periodontal clinical parameters. Circulating levels of 12 DP-related SASP factors were analyzed by immunoassay. Correlation between periodontal clinical parameters and circulating SASP levels was analyzed by Spearman's rank correlation coefficient and back propagation artificial neural network (BPNN). Senescence markers (p16, p21, and HMGB1) in gingiva were determined by immunofluorescence assay. ResultsM-DP group had increased serum levels of twelve SASP factors compared with the M-P group (p < 0.5). Serum levels of IL-6, IL-4, and RAGE were higher in the A-DP group than the A-P group (p < 0.5). The circulating concentrations of certain SASP proteins, including IL-1 beta, IL-4, MMP-8, OPG, RANKL, and RAGE were correlated with the clinical parameters of DP. BPNN showed that serum SASP levels had considerable predictive value for CAL of DP. Additionally, the DP group had higher expressions of p16, p21, and cytoplasmic-HMGB1 in the gingiva than the P group (p < 0.5). ConclusionsSignificantly enhanced circulating SASP levels and aggravated periodontal destruction were observed in patients with DP. Importantly, a non-negligible association between serum SASP levels and the severity of DP was found.
引用
收藏
页码:986 / 996
页数:11
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