Two ARGONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis

被引：12

作者：

Bradamante, Gabriele ^{[1
]}

Nguyen, Vu Hoang ^{[1
]}

Incarbone, Marco ^{[1
,4
]}

Meir, Zohar ^{[2
,3
]}

Bente, Heinrich ^{[1
]}

Dona, Mattia ^{[1
]}

Lettner, Nicole ^{[1
]}

Scheid, Ortrun Mittelsten ^{[1
]}

Gutzat, Ruben ^{[1
]}

机构：

[1] Austrian Acad Sci, Gregor Mendel Inst Mol Plant Biol, Vienna Bioctr VBC, A-1030 Vienna, Austria

[2] Weizmann Inst Sci, Fac Math & Comp Sci, IL-7610001 Rehovot, Israel

[3] Weizmann Inst Sci, Dept Plant & Environm Sci, IL-7610001 Rehovot, Israel

[4] Max Planck Inst Mol Plant Physiol, Golm, Germany

来源：

PLANT CELL | 2024年

基金：

奥地利科学基金会;

关键词：

DNA METHYLATION; GENOME DOSAGE; CHROMATIN; EXPRESSION; ELEMENTS; GERMLINE; THALIANA; MUTANTS; PROMOTE; SIRNAS;

D O I：

10.1093/plcell/koad295

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In sexually propagating organisms, genetic, and epigenetic mutations are evolutionarily relevant only if they occur in the germline and are hence transmitted to the next generation. In contrast to most animals, plants are considered to lack an early segregating germline, implying that somatic cells can contribute genetic information to progeny. Here we demonstrate that 2 ARGONAUTE proteins, AGO5 and AGO9, mark cells associated with sexual reproduction in Arabidopsis (Arabidopsis thaliana) throughout development. Both AGOs are loaded with dynamically changing small RNA populations derived from highly methylated, pericentromeric, long transposons. Sequencing of single stem cell nuclei revealed that many of these transposons are co-expressed within an AGO5/9 expression domain in the shoot apical meristem (SAM). Co-occurrence of transposon expression and specific ARGONAUTE (AGO) expression in the SAM is reminiscent of germline features in animals and supports the existence of an early segregating germline in plants. Our results open the path to investigating transposon biology and epigenome dynamics at cellular resolution in the SAM stem cell niche. Transposons are expressed in a subset of SAM stem cells, processed into small RNAs, and loaded onto AGO proteins to influence DNA methylation and likely transposon activity.

引用

页码：863 / 880

页数：18

共 88 条

[51] Arabidopsis ARGONAUTE 1 Binds Chromatin to Promote Gene Transcription in Response to Hormones and Stresses [J].