Comprehensive study on the effect of dietary leucine supplementation on intestinal physiology, TOR signaling and microbiota in juvenile turbot (Scophthalmus maximus L.)

被引:5
作者
Sui, Zhongmin [1 ,3 ]
Wang, Ning [1 ,3 ]
Zhang, Xiaojing [1 ,3 ]
Liu, Chengdong [1 ,3 ,4 ]
Wang, Xuan [1 ,3 ]
Zhou, Huihui [1 ,3 ]
Mai, Kangsen [1 ,3 ]
He, Gen [1 ,2 ,3 ]
机构
[1] Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266003, Peoples R China
[3] Ocean Univ China, Key Lab Aquaculture Nutr & Feeds, Minist Agr, Qingdao 266003, Peoples R China
[4] 5 Yushan Rd, Qingdao 266003, Peoples R China
关键词
Leucine; Intestinal health; TOR signaling pathway; Intestinal immune response; Intestinal microbiota; ANTIOXIDANT ENZYMES; IMMUNE-RESPONSES; BARRIER FUNCTION; CELL MIGRATION; NITRIC-OXIDE; AMINO-ACIDS; ARGININE; EXPRESSION; PATHWAY; NUTRITION;
D O I
10.1016/j.fsi.2023.109060
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Intestinal damage and inflammation are major health and welfare issues in aquaculture. Considerable efforts have been devoted to enhancing intestinal health, with a specific emphasis on dietary additives. Branch chain amino acids, particularly leucine, have been reported to enhance growth performance in various studies. However, few studies have focused on the effect of leucine on the intestinal function and its underlying molecular mechanism is far from fully illuminated. In the present study, we comprehensively evaluated the effect of dietary leucine supplementation on intestinal physiology, signaling transduction and microbiota in fish. Juvenile turbot (Scophthalmus maximus L.) (10.13 +/- 0.01g) were fed with control diet (Con diet) and leucine supplementation diet (Leu diet) for 10 weeks. The findings revealed significant improvements in intestinal morphology and function in the turbot fed with Leu diet. Leucine supplementation also resulted in a significant increase in mRNA expression levels of mucosal barrier genes, indicating enhanced intestinal integrity. The transcriptional levels of pro-inflammatory factors il-1 beta, tnf-alpha and irf-1 was decreased in response to leucine supplementation. Conversely, the level of anti-inflammatory factors tgf-beta, il-10 and nf-kappa b were up-regulated by leucine supplementation. Dietary leucine supplementation led to an increase in intestinal complement (C3 and C4) and immunoglobulin M (IgM) levels, along with elevated antioxidant activity. Moreover, dietary leucine supplementation significantly enhanced the postprandial phosphorylation level of the target of rapamycin (TOR) signaling pathway in the intestine. Finally, intestinal bacterial richness and diversity were modified and intestinal bacterial composition was re-shaped by leucine supplementation. Overall, these results provide new insights into the beneficial role of leucine supplementation in promoting intestinal health in turbot, offering potential implications for the use of leucine as a nutritional supplement in aquaculture practices.
引用
收藏
页数:11
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