Simultaneous Analysis of Cyanotoxins β-N-methylamino-L-alanine (BMAA) and Microcystins-RR, -LR, and -YR Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

被引:7
作者
Pravadali-Cekic, Sercan [1 ]
Vojvodic, Aleksandar [1 ]
Violi, Jake P. [2 ]
Mitrovic, Simon M. [3 ]
Rodgers, Kenneth J. [3 ]
Bishop, David P. [1 ]
机构
[1] Univ Technol Sydney, Hyphenated Mass Spectrometry Lab HyMAS, Sydney, NSW 2007, Australia
[2] Univ New South Wales, Sch Chem, Sydney, NSW 2033, Australia
[3] Univ Technol Sydney, Sch Life Sci, Sydney, NSW 2007, Australia
来源
MOLECULES | 2023年 / 28卷 / 18期
关键词
BMAA; microcystins; cyanotoxins; cyanobacteria; method validation; FRESH-WATER CYANOBACTERIA; NEUROTOXIN BMAA; NATURAL-WATER; AMINO-ACIDS; TOXINS; ISOMERS; SAMPLES; HEPATOTOXINS; NODULARIN; SHELLFISH;
D O I
10.3390/molecules28186733
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
beta-N-methylamino-L-alanine (BMAA) and its isomers, 2,4-diaminobutyric acid (2,4-DAB) and N-(2-aminoethyl)-glycine (AEG), along with microcystins (MCs)-RR, -LR, and -YR (the major MC congeners), are cyanotoxins that can cause detrimental health and environmental impacts during toxic blooms. Currently, there are no reverse-phase (RP) LC-MS/MS methods for the simultaneous detection and quantification of BMAA, its isomers, and the major MCs in a single analysis; therefore, multiple analyses are required to assess the toxic load of a sample. Here, we present a newly developed and validated method for the detection and quantification of BMAA, 2,4-DAB, AEG, MC-LR, MC-RR, and MC-YR using RP LC-MS/MS. Method validation was performed, assessing linearity (r(2) > 0.996), accuracy (>90% recovery for spiked samples), precision (7% relative standard deviation), and limits of detection (LODs) and quantification (LOQs) (ranging from 0.13 to 1.38 ng mL(-1)). The application of this combined cyanotoxin analysis on a culture of Microcystis aeruginosa resulted in the simultaneous detection of 2,4-DAB (0.249 ng mg(-1) dry weight (DW)) and MC-YR (4828 ng mg(-1) DW). This study provides a unified method for the quantitative analysis of BMAA, its isomers, and three MC congeners in natural environmental samples.
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页数:17
相关论文
共 80 条
[1]   Methods for simultaneous detection of the cyanotoxins BMAA, DABA, and anatoxin-α in environmental samples [J].
Al-Sammak, Maitham Ahmed ;
Hoagland, Kyle D. ;
Snow, Daniel D. ;
Cassada, David .
TOXICON, 2013, 76 :316-325
[2]  
Ananya A.K., 2014, Int J Innov Appl Stud, V7, P251
[3]  
[Anonymous], 2020, Cyanobacterial toxins: microcystins
[4]  
[Anonymous], WaterNSW Algae
[5]   Multiclass cyanotoxin analysis in reservoir waters: Tandem solid-phase extraction followed by zwitterionic hydrophilic interaction liquid chromatography-mass spectrometry [J].
Aparicio-Muriana, M. Mar ;
Carmona-Molero, Rocio ;
Lara, Francisco J. ;
Garcia-Campana, Ana M. ;
del Olmo-Iruela, Monsalud .
TALANTA, 2022, 237
[6]  
Australian Government Initiative, Cyanobacteria (Blue-Green Algae) and Water Quality
[7]  
Australian Water Quality Centre, Algal Toxin Tests
[8]  
AWWA, Cyanotoxins in US Drinking Water: Occurrence, Case Studies and State Approaches to Regulation
[9]   1ST REPORT OF MICROCYSTINS FROM A BRAZILIAN ISOLATE OF THE CYANOBACTERIUM MICROCYSTIS-AERUGINOSA [J].
AZEVEDO, SMFO ;
EVANS, WR ;
CARMICHAEL, WW ;
NAMIKOSHI, M .
JOURNAL OF APPLIED PHYCOLOGY, 1994, 6 (03) :261-265
[10]   Assessing Environmental Exposure to β-N-Methylamino-L-Alanine (BMAA) in Complex Sample Matrices: a Comparison of the Three Most Popular LC-MS/MS Methods [J].
Baker, Teesha C. ;
Tymm, Fiona J. M. ;
Murch, Susan J. .
NEUROTOXICITY RESEARCH, 2018, 33 (01) :43-54