Phytosphingosine alleviates Staphylococcus aureus-induced mastitis by inhibiting inflammatory responses and improving the blood-milk barrier in mice

被引：9

作者：

Zhao, Yihong ^{[1
]}

Xu, Jiawen ^{[1
]}

Zhao, Caijun ^{[1
]}

Bao, Lijuan ^{[1
]}

Wu, Keyi ^{[1
]}

Feng, Lianjun ^{[1
]}

Sun, Hao ^{[1
]}

Shang, Shan ^{[1
]}

Hu, Xiaoyu ^{[1
]}

Sun, Qingsong ^{[2
]}

Fu, Yunhe ^{[1
]}

机构：

[1] Jilin Univ, Coll Vet Med, Dept Clin Vet Med, Changchun 130062, Jilin, Peoples R China

[2] Jilin Agr Sci & Technol Univ, Coll Anim Sci & Technol, Key Lab Prevent Vet Med Jilin Prov, Jilin 132101, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2023年 / 182卷

关键词：

Phytosphingosine; Mastitis; Staphylococcus aureus; Blood-milk barrier; SKIN BARRIER; FATTY-ACIDS; SPHINGOLIPIDS; METABOLISM;

D O I：

10.1016/j.micpath.2023.106225

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mastitis is one of the common diseases in dairy cows which threatens the health of cows and impacts on eco-nomic benefits seriously. Recent studies have been showed that Subacute Ruminal Acidosis (SARA) increased the susceptibility of cow mastitis. SARA leads the disturbance of the rumen microbiota, and the rumen bacterial disordered community is an important endogenous factor of cow mastitis. That is to say, cows which suffer from SARA have a disordered rumen microbiota, a prolonged decline in ruminal PH and a high level of lipopoly-saccharide (LPS) in the rumen, blood. Therefore, ruminal metabolism is closely related to the rumen microbiota. However, the specific mechanism of SARA and mastitis still not clear. We found an intestinal metabolite ac-cording to the metabonomics, which is correlated to inflammation. Phytophingosine (PS), a product from rumen fluid and milk of the cows which suffer from SARA and mastitis. It has the effect of killing bacteria and anti-inflammatory. Emerging evidences indicate that PS can alleviate inflammatory diseases. However, how PS af-fects mastitis is largely unknown. In this study, we explored the concrete role of PS on Staphylococcus aureus (S. aureus)-induced mastitis in mice. We found that PS obviously decreased the level of the proinflammatory cytokines. Meanwhile, PS also significantly relieved the mammary gland inflammation caused by S. aureus and restored the function of the blood-milk barrier. Here, we showed that PS increased the expression of the classic Tight-junctions (TJs) proteins including ZO-1, Occludin and Claudin-3. Moreover, PS improves S. aureus-induced mastitis by inhibiting the activation of the NF-& kappa;B and NLRP3 signaling pathways. These data indicated that PS relieved S. aureus-induced mastitis effectively. This also provides a reference for exploring the correlation be-tween the intestinal metabolism and inflammation.

引用

页数：7

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