Structure and phase separation of the C-terminal domain of RNA polymerase II

被引：2

作者：

Lushpinskaia, Irina P. P. ^{[1
]}

Flores-Solis, David ^{[1
]}

Zweckstetter, Markus ^{[1
,2
]}

机构：

[1] German Ctr Neurodegenerat Dis DZNE, Von Siebold Str 3A, D-35075 Gottingen, Germany

[2] Max Planck Inst Multidisciplinary Sci, Dept NMR Based Struct Biol, Fassberg 11, D-37077 Gottingen, Germany

来源：

BIOLOGICAL CHEMISTRY | 2023年 / 404卷 / 8-9期

关键词：

CTD; liquid-liquid phase separation; RNA Pol II; transcription; CTD; REPEAT;

D O I：

10.1515/hsz-2023-0136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The repetitive heptads in the C-terminal domain (CTD) of RPB1, the largest subunit of RNA Polymerase II (Pol II), play a critical role in the regulation of Pol II-based transcription. Recent findings on the structure of the CTD in the pre-initiation complex determined by cryo-EM and the novel phase separation properties of key transcription components offers an expanded mechanistic interpretation of the spatiotemporal distribution of Pol II during transcription. Current experimental evidence further suggests an exquisite balance between CTD's local structure and an array of multivalent interactions that drive phase separation of Pol II and thus shape its transcriptional activity.

引用

页码：839 / 844

页数：6

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