Optimization of Ursolic Acid Extraction in Oil from Annurca Apple to Obtain Oleolytes with Potential Cosmeceutical Application

被引：20

作者：

Maisto, Maria ^{[1
]}

Piccolo, Vincenzo ^{[1
]}

Novellino, Ettore ^{[2
]}

Schiano, Elisabetta ^{[1
]}

Iannuzzo, Fortuna ^{[1
]}

Ciampaglia, Roberto ^{[1
]}

Summa, Vincenzo ^{[1
]}

Tenore, Gian Carlo ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Pharm, Via Domen Montesano 49, I-80131 Naples, Italy

[2] Univ Cattolica Sacro Cuore, Fac Med, Largo Francesco Vito, I-00168 Rome, Italy

来源：

ANTIOXIDANTS | 2023年 / 12卷 / 02期

关键词：

ursolic acid; oleolyte; antiaging; response surface methodology; biocompatible solvent; ANTIOXIDANT; FRUIT; POLYPHENOLS; QUANTIFICATION; IDENTIFICATION; CONSTITUENTS; TRITERPENE;

D O I：

10.3390/antiox12020224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ursolic acid (UA) is a plant-derived molecule with relevant anti-aging activity, which makes this molecule a potential functional active ingredient in cosmetic formulations. The main objectives of this study were to optimize the UA extraction process from Annurca apple (AA) with sunflower oil as a lyophilic food-grade solvent using Response Surface Methodology (RSM) to determine the potential cosmetic application of the obtained extract. The results of RSM analysis showed a maximum UA yield of 784.40 +/- 7.579 (mu g/mL) obtained under the following optimized conditions: sunflower oil as extraction solvent, 68.85 degrees C as extraction temperature, and 63 h as extraction time. The HPLC-DAD-HESI-MS/MS analysis performed on the extract obtained under these conditions, named Optimized Annurca Apple Oleolyte (OAAO), led to the identification of twenty-three phenolic and terpenoid molecules and the quantification of eight of them. To explore the biological properties of OAAO, the in vitro antioxidant activity was evaluated by DPPH, ABTS, and FRAP assays, resulting in 16.63 +/- 0.22, 5.90 +/- 0.49, and 21.72 +/- 0.68 mu mol Trolox equivalent/g extract, respectively. Moreover, the permeation study has shown that OAAO may be considered a safe and functional ingredient in potential cosmetic formulations.

引用

页数：19

共 53 条

[11] Extraction optimization of oleanolic and ursolic acids from pomegranate (Punica granatum L.) flowers [J].