Early resource scarcity causes cortical astrocyte enlargement and sex-specific changes in the orbitofrontal cortex transcriptome in adult rats

被引:3
作者
Deckers, Claire [1 ]
Karbalaei, Reza [1 ]
Miles, Nylah A. [1 ]
Harder, Eden V. [2 ]
Witt, Emily [2 ]
Harris, Erin P. [3 ,4 ]
Reissner, Kathryn [2 ]
Wimmer, Mathieu E. [1 ]
Bangasser, Debra A. [1 ,3 ,4 ,5 ]
机构
[1] Temple Univ, Dept Psychol & Neurosci Program, Philadelphia, PA USA
[2] Univ North Carolina Chapel Hill, Dept Psychol & Neurosci, Chapel Hill, NC USA
[3] Georgia State Univ, Neurosci Inst, Atlanta, GA USA
[4] Georgia State Univ, Ctr Behav Neurosci, Atlanta, GA USA
[5] Georgia State Univ, 100 Piedmont Ave,SE, Atlanta, GA 30303 USA
来源
NEUROBIOLOGY OF STRESS | 2024年 / 29卷
基金
美国国家科学基金会;
关键词
Glia; Stress; Morphology; Glutamate; Sex differences; Cortex; FIBRILLARY ACIDIC PROTEIN; MAJOR DEPRESSIVE DISORDER; EARLY-LIFE STRESS; PREFRONTAL CORTEX; ENRICHMENT ANALYSIS; EXPRESSION; GENE; IMMUNOREACTIVITY; TRANSMISSION; HIPPOCAMPUS;
D O I
10.1016/j.ynstr.2024.100607
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Astrocyte morphology affects function, including the regulation of glutamatergic signaling. This morphology changes dynamically in response to the environment. However, how early life manipulations alter adult cortical astrocyte morphology is underexplored. Our lab uses brief postnatal resource scarcity, the limited bedding and nesting (LBN) manipulation, in rats. We previously found that LBN augments maternal behaviors and promotes later resilience to adult addiction-related behaviors, reducing impulsivity, risky decision-making, and morphine self-administration. These behaviors rely on glutamatergic transmission in the medial orbitofrontal (mOFC) and medial prefrontal (mPFC) cortex. Here we tested whether LBN changed astrocyte morphology in the mOFC and mPFC of adult rats using a novel viral approach that, unlike traditional markers, fully labels astrocytes. Prior exposure to LBN causes an increase in the surface area and volume of astrocytes in the mOFC and mPFC of adult males and females relative to control-raised rats. We next used bulk RNA sequencing of OFC tissue to assess transcriptional changes that could increase astrocyte size in LBN rats. LBN caused mainly sex-specific changes in differentially expressed genes. Pathway analysis revealed that OFC glutamatergic signaling is altered by LBN in males and females, but the gene changes in that pathway differed across sex. This may represent a convergent sex difference where glutamatergic signaling, which affects astrocyte morphology, is altered by LBN via sex-specific mechanisms. Collectively, these studies highlight that astrocytes may be an important cell type that mediates the effect of early resource scarcity on adult brain function.
引用
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页数:12
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