miRNAs: novel noninvasive biomarkers as diagnostic and prognostic tools in neonatal sepsis

被引：9

作者：

El-Khazragy, Nashwa ^{[1
,2
,3
,4
]}

Mohamed, Noura Mostafa ^{[5
,6
]}

Mostafa, Mohamed Fathalla ^{[7
]}

Elnakib, Mostafa ^{[8
]}

Hemida, Eman H. A. ^{[9
]}

Salah, Amira ^{[10
]}

Fawzy, Nesma Mohamed ^{[10
]}

Safwat, Gehan ^{[11
]}

Emam, Mennatallah M. ^{[11
]}

Mahran, Nievin Ahmed ^{[12
]}

Rabie, Dina ^{[7
]}

机构：

[1] Ain Shams Univ, Dept Clin Pathol Hematol, Fac Med, Cairo, Egypt

[2] Ain Shams Univ, Ain Shams Med Res Inst MASRI, Fac Med, Cairo, Egypt

[3] Egypt Ctr Res & Regenerat Med ECRRM, Dept Genet & Mol Biol, Cairo, Egypt

[4] Armed Forces Coll Med AFCM, Dept Med Res, Cairo, Egypt

[5] Zagazig Univ, Fac Med, Dept Med Biochem, Zagazig, Egypt

[6] Princess Nourah Bint Abdulrahman Univ PNU, Fac Preparatory Year Hlth Sci, Dept Sci, Riyadh, Saudi Arabia

[7] Ain Shams Univ, Fac Med, Dept Pediat, Cairo, Egypt

[8] Mil Med Acad, Dept Med Microbiol & Immunol, Cairo, Egypt

[9] Ain Shams Univ, Fac Sci, Dept Biochem, Cairo, Egypt

[10] Ain Shams Univ, Fac Med, Dept Med Biochem & Mol Biol, Cairo, Egypt

[11] October Univ Modern Sci & Arts MSA, Fac Biotechnol, Giza, Egypt

[12] Sinai Univ, Fac Dent, Biochem Dept, Kanatra, Egypt

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 107卷 / 03期

关键词：

miRNA; miR-1; miR-124; miR-34a; mortality; neonatal sepsis; ONSET SEPSIS;

D O I：

10.1016/j.diagmicrobio.2023.116053

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The study aimed to assess the diagnostic and prognostic value of 3 specific microRNAs (miRNAs) in early-onset neonatal sepsis (NS). We examined miR-1, miR-124, and miR-34a in 70 NS patients upon admission and compared them to 70 healthy controls by RT-PCR. The main finding of the study was the difference in miRNA expression levels between NS patients and controls. Higher expression levels of miR-1 and miR-124 were significantly associated with NS, while miR34a expression was reduced. Among the studied miRNAs, miR-34a exhibited the highest specificity (97%) as a confirmatory test for NS. In the multivariate model, miR-1 and miR-124 were found to be significant predictors of disease progression or mortality. Overall, the study suggests that miR-1, miR-124, and miR-34a could serve as potential biomarkers for diagnosing and predict-ing outcomes in early-onset NS. & COPY; 2023 Elsevier Inc. All rights reserved.

引用

页数：8

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