Determination of specific autoantibodies in patients with systemic lupus erythematosus by Line immunoassay, ELISA and CLIF

被引：1

作者：

Wongjarit, Kanphai ^{[1
]}

Thammacharoenrach, Niramol ^{[2
]}

Dityen, Kanthaporn ^{[2
]}

Kaewopas, Yadah ^{[2
]}

Kositpesat, Naravadee ^{[3
]}

Ukritchon, Sittichai ^{[4
]}

Osiri, Manathip ^{[4
]}

Wongpiyabovorn, Jongkonnee ^{[5
,6
]}

机构：

[1] Chulalongkorn Univ, Fac Med, Dept Microbiol, Bangkok, Thailand

[2] Chulalongkorn Univ, Fac Med, Dept Microbiol, Div Immunol, Bangkok, Thailand

[3] Bangkok Hosp Hua Hin, Internal Med & Rheumatol, Prachuap Khirikhan, Thailand

[4] Chulalongkorn Univ, Fac Med, Dept Med, Div Rheumatol, Bangkok, Thailand

[5] Chulalongkorn Univ, Fac Med, Ctr Excellence Immunol & Immune Mediated Dis, Dept Microbiol, Bangkok, Thailand

[6] Chulalongkorn Univ, Div Immunol, Dept Microbiol, Fac Med, Rama 4 Rd, Bangkok 10330, Thailand

来源：

ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY | 2023年 / 41卷 / 01期

关键词：

Systemic lupus erythematosus (SLE); Specific antinuclear antibodies; Line immunoassay (LIA); Enzyme-linked immunosorbent assay (ELISA); Crithidia luciliae indirect immunofluorescence assay (CLIF); DISEASE-ACTIVITY INDEX; CLASSIFICATION CRITERIA; AMERICAN-COLLEGE; RHEUMATOLOGY/EUROPEAN LEAGUE; REVISED CRITERIA; ASSAYS; ANTIBODIES; VALIDATION; GUIDELINES; DERIVATION;

D O I：

10.12932/ap-301019-0681

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Detection of specific antinuclear antibodies (ANA) is very importance in term of diagnosis, prognosis and management of patients with systemic lupus erythematosus (SLE). To date, Line immunoassay (LIA), enzyme-linked immunosorbent assay (ELISA) and Crithidia luciliae indirect immunofluorescence assay (CLIF) are commonly used for detection of specific ANA.Objective: To determine the performance of LIA, ELISA and CLIF for the detection of anti-double-stranded DNA (dsDNA), anti-nucleosome, and anti-extractable nuclear antigens (ENA) antibodies in patients with SLE.Methods: A total 100 sera from 50 patients with SLE, 25 patients with disease control and 25 healthy control subjects were tested for anti-dsDNA, anti-nucleosome, and anti-ENA antibodies by LIA, ELISA, and CLIF. Agreement and diagnostic performance of each assay were analyzed using Cohen's kappa coefficient and receiver operating characteristic curve analysis.Results: For the detection of anti-dsDNA antibody, ELISA had a substantial agreement with CLIF (kappa = 0.74) but LIA had a fair agreement with ELISA and CLIF (kappa = 0.37, and 0.35 respectively). For the detection of anti-nucleosome, anti-nRNP/Sm, anti-Sm, anti-SSA, and anti-SSB antibodies, LIA had a substantial to perfect agreement with ELISA (kappa = 0.64, 0.78, 0.68, 0.91, and 0.74, respectively). Anti-dsDNA-NcX ELISA and anti-dsDNA CLIF had equally diagnostic performance (sensitivity, 66% vs. 68%, and specificity, 96% vs. 94%, respectively) whereas, anti-dsDNA LIA has low sensitivity (22%) but high specificity (100%).Conclusion: LIA, ELISA, and CLIF demonstrated comparable performance for the detection of specific antinuclear-antibodies. However, there were some discrepancy between assays particularly in the detection of anti-dsDNA antibody.

引用

页码：73 / 79

页数：7

共 27 条

[1] International recommendations for the assessment of autoantibodies to cellular antigens referred to as anti-nuclear antibodies [J].

Agmon-Levin, Nancy ;

Damoiseaux, Jan ;

Kallenberg, Cees ;

Sack, Ulrich ;

Witte, Torsten ;

Herold, Manfred ;

Bossuyt, Xavier ;

Musset, Lucille ;

Cervera, Ricard ;

Plaza-Lopez, Aresio ;

Dias, Carlos ;

Sousa, Maria Jose ;

Radice, Antonella ;

Eriksson, Catharina ;

Hultgren, Olof ;

Viander, Markku ;

Khamashta, Munther ;

Regenass, Stephan ;

Coelho Andrade, Luis Eduardo ;

Wiik, Allan ;

Tincani, Angela ;

Ronnelid, Johan ;

Bloch, Donald B. ;

Fritzler, Marvin J. ;

Chan, Edward K. L. ;

Garcia-De la Torre, I. ;

Konstantinov, Konstantin N. ;

Lahita, Robert ;

Wilson, Merlin ;

Vainio, Olli ;

Fabien, Nicole ;

Sinico, Renato Alberto ;

Meroni, Pierluigi ;

Shoenfeld, Yehuda .

ANNALS OF THE RHEUMATIC DISEASES, 2014, 73 (01) :17-23

[2]

Aletaha D, 2010, ANN RHEUM DIS, V69, P1580, DOI [10.1136/ard.2010.138461, 10.1002/art.27584]

[3]

Aringer M, 2019, ARTHRITIS RHEUMATOL, V71, P1400, DOI [10.1136/annrheumdis-2018-214819, 10.1002/art.40930]

[4] Anti-dsDNA-NcX ELISA: dsDNA-loaded nucleosomes improve diagnosis and monitoring of disease activity in systemic lupus erythematosus [J].

Biesen, Robert ;

Daehnrich, Cornelia ;

Rosemann, Anke ;

Barkhudarova, Fidan ;

Rose, Thomas ;

Jakob, Olga ;

Bruns, Anne ;

Backhaus, Marina ;

Stoecker, Winfried ;

Burmester, Gerd-Ruediger ;

Schlumberger, Wolfgang ;

Egerer, Karl ;

Hiepe, Falk .

ARTHRITIS RESEARCH & THERAPY, 2011, 13 (01)

[5] POLYMYOSITIS AND DERMATOMYOSITIS .1. [J].

BOHAN, A ;

PETER, JB .

NEW ENGLAND JOURNAL OF MEDICINE, 1975, 292 (07) :344-347

[6] DERIVATION OF THE SLEDAI - A DISEASE-ACTIVITY INDEX FOR LUPUS PATIENTS [J].

BOMBARDIER, C ;

GLADMAN, DD ;

UROWITZ, MB ;

CARON, D ;

CHANG, CH .

ARTHRITIS AND RHEUMATISM, 1992, 35 (06) :630-640

[7] From autoantibody research to standardized diagnostic assays in the management of human diseases - report of the 12th Dresden Symposium on Autoantibodies [J].

Conrad, K. ;

Andrade, L. E. C. ;

Chan, E. K. L. ;

Mahler, M. ;

Meroni, P. L. ;

Pruijn, G. J. M. ;

Steiner, G. ;

Shoenfeld, Y. .

LUPUS, 2016, 25 (08) :787-796

[8] Evaluation of a novel line-blot immunoassay for the detection of antibodies to extractable nuclear antigens [J].

Damoiseaux, J ;

Boesten, K ;

Giesen, J ;

Austen, J ;

Tervaert, JWC .

AUTOIMMUNITY: CONCEPTS AND DIAGNOSIS AT THE CUTTING EDGE, 2005, 1050 :340-347

[9] Reactivity in ELISA with DNA-loaded nucleosomes in patients with proliferative lupus nephritis [J].

Dieker, Jurgen ;

Schlumberger, Wolfgang ;

McHugh, Neil ;

Hamann, Philip ;

van der Vlag, Johan ;

Berden, Jo H. .

MOLECULAR IMMUNOLOGY, 2015, 68 (01) :20-24

[10] Four Anti-dsDNA Antibody Assays in Relation to Systemic Lupus Erythematosus Disease Specificity and Activity [J].

Enocsson, Helena ;

Sjowall, Christopher ;

Wirestam, Lina ;

Dahle, Charlotte ;

Kastbom, Alf ;

Ronnelid, Johan ;

Wettero, Jonas ;

Skogh, Thomas .

JOURNAL OF RHEUMATOLOGY, 2015, 42 (05) :817-825

← 1 2 3 →