The effect of Brazilian Green Propolis extract on inflammation in patients with chronic kidney disease on peritoneal dialysis: A randomised double-blind controlled clinical trial

被引:21
作者
Baptista, Beatriz Germer [1 ]
Fanton, Susane [2 ]
Ribeiro, Marcia [3 ]
Cardozo, Ludmila F. M. F. [2 ]
Regis, Bruna [2 ]
Alvarenga, Livia [1 ,3 ]
Ribeiro-Alves, Marcelo [4 ]
Berretta, Andresa A. [5 ]
Shiels, Paul G. [6 ]
Mafra, Denise [1 ,3 ]
机构
[1] Fluminense Fed Univ UFF, Grad Program Med Sci, Niteroi, RJ, Brazil
[2] Fluminense Fed Univ UFF, Grad Program Cardiovasc Sci, Niteroi, RJ, Brazil
[3] Fed Univ Rio de Janeiro UFRJ, Grad Program Biol Sci Physiol, Rio De Janeiro, RJ, Brazil
[4] Natl Inst Infectol INI Fiocruz, HIV AIDS Clin Res Ctr, Rio De Janeiro, RJ, Brazil
[5] Apis Flora Indl Coml Ltda, Res Dev & Innovat Dept, Ribeirao Preto, SP, Brazil
[6] Univ Glasgow, Wolfson Wohl Translat Res Ctr, Garscube Estate,Switchback Rd, Glasgow G61 1QH, Scotland
关键词
Propolis; Inflammation; Chronic kidney disease; Oxidative stress; Peritoneal dialysis; NF-KAPPA-B; CHEMICAL-COMPOSITION; PATHWAYS; ORIGIN; SYSTEM; TRENDS;
D O I
10.1016/j.phymed.2023.154731
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Chronic kidney disease (CKD) patients on dialysis display a low-grade systemic inflammatory burden. Nutritional interventions designed to activate the cytoprotective nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibit nuclear factor-kB (NF-kappa B) have been proposed to mitigate this burden. Several bioactive compounds have been investigated to achieve this, including propolis, a resin produced by Apis mellifera bees. Considering the safety and efficacy of propolis, it could be a strategy to benefit these patients. Still, there are no studies using propolis in patients with CKD on peritoneal dialysis (DP), and clinical studies to support this application are lacking. Hypothesis/Purpose: The objective and novelty of the present study are to evaluate the effects of propolis sup-plementation on inflammatory markers in patients with CKD on PD. Study design: A longitudinal, double-blind, placebo-controlled trial with CKD patients on PD. Methods: The patients were randomised into two groups: propolis that received four capsules of 100 mg (400 mg/ day), containing concentrated and standardised dry EPP-AF((R)) Brazilian green propolis extract) or placebo, four capsules of 100 mg (400 mg/day), of magnesium stearate, silicon dioxide, and microcrystalline cellulose, for two months. Plasma levels of inflammatory cytokines, including tumour necrosis factor (TNF-alpha) and interleukin-6 (IL-6), were evaluated by ELISA. Quantitative real-time PCR analyses were performed to evaluate the transcriptional expression levels of Nrf2 and NF-kappa B in peripheral blood mononuclear cells (PBMCs). Plasma malondialdehyde (MDA) levels, a lipid peroxidation marker, was measured as thiobarbituric acid reactive substances (TBARS). Routine biochemical markers, including C-reactive protein (CRP), were analysed using commercial kits. Carotid Intima-Media Thickness (CIMT) was measured with a doppler ultrasonography device. The study was registered on ClinicalTrials.gov under the number NCT04411758.Results: A total of 19 patients completed the study, ten patients in the propolis group (54 +/- 1.0 years, five men, 7.2 (5.1) months on PD) and 9 in the placebo group (47.5 +/- 15.2 years, three men, 10.8 (24.3) months on PD). The plasma levels of TNF-alpha reduced significantly (p = 0.02), and expression of Nrf2 showed a trend to increase (p = 0.07) after propolis supplementation.Conclusion: EPP-AF((R)) Green Propolis extract (400 mg/day) supplementation for two months appears as a po-tential strategy to mitigate inflammation, reducing TNF-alpha plasma levels in CKD patients on PD.
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页数:9
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