Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization

被引:4
作者
Pentek, Sandor [1 ,2 ]
Varnagy, Akos [1 ,2 ,3 ]
Farkas, Balint [1 ,2 ,3 ]
Mauchart, Peter [1 ,2 ,3 ]
Godony, Krisztina [1 ,2 ,3 ]
Varjas, Timea [1 ,4 ]
Koszegi, Tamas [1 ,5 ]
Kaltenecker, Peter [1 ,5 ]
Jakabfi-Csepregi, Rita [1 ,5 ]
Kovacs, Kalman [1 ,2 ,3 ]
Bodis, Jozsef [1 ,2 ,3 ]
Sulyok, Endre [1 ,6 ]
机构
[1] Univ Pecs, Natl Lab Human Reprod, H-7622 Pecs, Hungary
[2] Univ Pecs, Med Sch, Dept Obstet & Gynecol, H-7624 Pecs, Hungary
[3] Univ Pecs, MTA PTE Human Reprod Sci Res Grp, H-7624 Pecs, Hungary
[4] Univ Pecs, Med Sch, Dept Publ Hlth Med, H-7624 Pecs, Hungary
[5] Univ Pecs, Szentagothai Res Ctr, H-7624 Pecs, Hungary
[6] Univ Pecs, Fac Hlth Sci, H-7621 Pecs, Hungary
关键词
telomere length; telomerase activity; oxidative DNA damage; in vitro fertilization; OXIDATIVE STRESS; DNA; SHELTERIN; QUALITY; OOCYTES; EMBRYOS; HEALTH;
D O I
10.3390/antiox12020419
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagnoses. Granulosa cells (GCs) and follicular fluid (FF) were analyzed simultaneously for telomere functions and for the marker of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit and Telomerase Activity Quantification qPCR Assay kit (Nucleotestbio, Budapest, Hungary), as well as an 8-OHdG ELISA kit (Abbexa Ltd., Cambridge, United Kingdom) were used for analyses. Similar telomere lengths were found in GCs and FF, however telomerase activity was markedly depressed, while 8-OHdG levels were markedly elevated in FF compared with those in GCs (p < 0.01). Telomere lengths were independent of telomerase activity both in GCs and FF. However, GC 8-OHdG was inversely related to telomerase activity in GCs and FF (p < 0.05). Importantly, 8-OHdG levels both in GCs and FF had significant negative impact on the number of the retrieved and MII oocytes (p < 0.01), whereas FF 8-OHdG was negatively related further to the number of fertilized oocytes and blastocysts (p < 0.01). In conclusion, we could not confirm the direct association of telomere function and reproductive potential. However, oxidative DNA damage, as mainly reflected by 8-OHdG, adversely affected early markers of IVF outcome and clinical pregnancies.
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页数:13
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