Myo-inositol improves the viability of boar sperm during liquid storage

被引:3
|
作者
Jawad, Ali [1 ,2 ,3 ]
Oh, Dongjin [1 ,2 ,3 ]
Choi, Hyerin [1 ,2 ,3 ]
Kim, Mirae [1 ,2 ,3 ]
Cai, Lian [1 ,2 ,3 ,4 ]
Lee, Joohyeong [1 ,2 ,3 ]
Hyun, Sang-Hwan [1 ,2 ,3 ,4 ]
机构
[1] Chungbuk Natl Univ, Vet Med Ctr, Lab Vet Embryol & Biotechnol VETEMBIO, Cheongju, South Korea
[2] Chungbuk Natl Univ, Coll Vet Med, Cheongju, South Korea
[3] Chungbuk Natl Univ, Inst Stem Cell & Regenerat Med ISCRM, Cheongju, South Korea
[4] Chungbuk Natl Univ, Grad Sch Vet Biosecur & Protect, Cheongju, South Korea
基金
新加坡国家研究基金会;
关键词
Myo-inositol; viability; boar; sperm; storage; MITOCHONDRIAL-FUNCTION; ASSISTED REPRODUCTION; PLASMA-MEMBRANE; SPERMATOZOA; EXPRESSION; MOTILITY; FERTILIZATION; INOSITOL; QUALITY; WOMEN;
D O I
10.3389/fvets.2023.1150984
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
IntroductionLiquid preservation of boar semen is a highly preferred method for semen preservation in pig production. However, oxidative stress is the main challenge during the liquid preservation of boar semen in a time dependent manner. Therefore, supplementation of sperm with antioxidants during storage to protect them from oxidative stress has been the focus of recent research. Myo-inositol (Myo-Ins), the most active form of inositol, which belongs to the vitamin (Vit.) (B1 group has been shown to improve semen quality) (1). This study aimed to investigate whether Myo-Ins supplementation protects boar sperm in liquid preservation against oxidative stress and determine the appropriate concentration of Myo-Ins to be used in this regard. MethodsBoar sperm was diluted with a semen extender with different concentrations of Myo-Ins (2, 4, 6, and 8 mg/mL) depending on the previous studies (1, 24). Sperm motility and viability, plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), semen time survival, and gene expression were measured and analyzed on days 0, 1, 3, 5, and 7 for the different samples. ResultsDifferent concentrations of Myo-Ins exerted different protective effects on the boar sperm quality. The addition of 2 mg/mL Myo-Ins resulted in higher sperm motility and viability, plasma membrane and acrosome integrity, MMP, and effective survival time. Investigation of mRNA expression patterns via qRT-PCR suggested that the 2 mg/mL Myo-Ins sample had increased expression of antioxidative genes. ConclusionThe addition of Myo-Ins to semen extender improved the boar semen quality by decreasing the effects of oxidative stress during liquid preservation at 17 & DEG;C. Additionally, 2 mg/mL is the optimum inclusion concentration of Myo-Ins for semen preservation.
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页数:11
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