Intestinal Colonization of Campylobacter jejuni and Its Hepatic Dissemination Are Associated with Local and Systemic Immune Responses in Broiler Chickens

被引:5
作者
Chagneau, Sophie [1 ,2 ]
Gaucher, Marie-Lou [1 ,2 ]
Fravalo, Philippe [3 ]
Theriault, William P. P. [1 ,2 ]
Thibodeau, Alexandre [1 ,2 ,4 ]
机构
[1] Univ Montreal, Fac Medecine Vet, Res Chair Meat Safety, Dept Pathol & Microbiol, St Hyacinthe, PQ J2S 2M2, Canada
[2] Univ Montreal, Fac Medecine Vet, Swine & Poultry Infect Dis Res Ctr, Dept Pathol & Microbiol, St Hyacinthe, PQ J2S 2M2, Canada
[3] Chaire Agroalimentaire Conservatoire Natl Arts & M, F-22440 Ploufragan, France
[4] Univ Montreal, Ctr Rech Sante Publ CReSP, Montreal, PQ H3N 1X9, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Campylobacter jejuni; broiler chickens; intestinal colonization; liver dissemination; cecal tonsils; local immune response; systemic immune response; serum amyloid A; anti-C; jejuni IgY antibodies; TOLL-LIKE RECEPTORS; CECAL TONSILS; EXPRESSION; INFECTION; CATHELICIDIN-2; OUTBREAKS; GENES;
D O I
10.3390/microorganisms11071677
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Campylobacter jejuni is an important foodborne pathogen. Despite the lack of clinical signs associated with its colonization in poultry, it has been reported to interact with the intestinal immune system. However, little is known about the interaction between C. jejuni and the chicken immune system, especially in the context of hepatic dissemination. Therefore, to follow up on our previous study showing intestinal colonization and hepatic spread of C. jejuni, cecal tonsils and liver samples were collected from these birds to determine the mRNA levels of chemokines and cytokines. Serum samples were also collected to determine serum amyloid A (SAA) concentrations and specific IgY titers. Lack of Th17 induction was observed in the cecal tonsils of only the liver-contaminated groups. This hepatic dissemination was accompanied by innate, Th1 and Th2 immune responses in livers, as well as an increase in SAA concentrations and specific IgY levels in sera. Campylobacter appears to be able to restrain the induction of the chicken gut immunity in particular conditions, possibly enhancing its hepatic dissemination and thus eliciting systemic immune responses. Although Campylobacter is often recognized as a commensal-like bacterium in chickens, it seems to modulate the gut immune system and induce systemic immunity.
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页数:17
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