Dorsal root ganglia P2X4 and P2X7 receptors contribute to diabetes-induced hyperalgesia and the downregulation of electroacupuncture on P2X4 and P2X7

被引：14

作者：

Hu, Qun-qi ^{[1
]}

He, Xiao-fen ^{[1
]}

Ma, Yi-qi ^{[1
]}

Ma, Li-qian ^{[1
]}

Qu, Si-ying ^{[1
]}

Wang, Han-zhi ^{[1
]}

Kang, Yu-rong ^{[1
]}

Chen, Lu-hang ^{[1
]}

Li, Xiang ^{[1
]}

Liu, Bo-yu ^{[1
]}

Shao, Xiao-mei ^{[1
]}

Fang, Jun-fan ^{[1
]}

Liang, Yi ^{[1
]}

Fang, Jian-qiao ^{[1
]}

Jiang, Yong-liang ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Clin Med Coll 3, Dept Neurobiol & Acupuncture Res, Key Lab Acupuncture & Neurol Zhejiang Prov, Hangzhou 310053, Zhejiang, Peoples R China

来源：

PURINERGIC SIGNALLING | 2023年 / 19卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Diabetic neuropathic pain; DRG; TNP-ATP; Electroacupuncture; NEUROPATHIC PAIN; ATP; ACTIVATION; CARE;

D O I：

10.1007/s11302-022-09844-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diabetic neuropathic pain (DNP) is highly common in diabetes patients. P2X receptors play critical roles in pain sensitization. We previously showed that elevated P2X3 expression in dorsal root ganglion (DRG) contributes to DNP. However, the role of other P2X receptors in DNP is unclear. Here, we established the DNP model using a single high-dose streptozotocin (STZ) injection and investigated the expression of P2X genes in the DRG. Our data revealed elevated P2X2, P2X4, and P2X7 mRNA levels in DRG of DNP rats. The protein levels of P2X4 and P2X7 in DNP rats increased, but the P2X2 did not change significantly. To study the role of P2X4 and P2X7 in diabetes-induced hyperalgesia, we treated the DNP rats with TNP-ATP (2',3'-O-(2,4,6-trinitrophenyl)-adenosine 5'-triphosphate), a nonspecific P2X1-7 antagonist, and found that TNP-ATP alleviated thermal hyperalgesia in DNP rats. 2 Hz electroacupuncture is analgesic against DNP and could downregulate P2X4 and P2X7 expression in DRG. Our findings indicate that P2X4 and P2X7 in L4-L6 DRGs contribute to diabetes-induced hyperalgesia, and that EA reduces thermal hyperalgesia and the expression of P2X4 and P2X7.

引用

页码：29 / 41

页数：13

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