Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine-derived stem cells

被引：0

作者：

Li, Shang ^{[1
,2
,3
,4
]}

Wang, Rui ^{[1
,2
]}

Huang, Liping ^{[1
,2
]}

Jiang, Yanlin ^{[1
,2
]}

Xing, Fei ^{[1
,2
]}

Duan, Weiqiang ^{[3
]}

Cen, Ying ^{[3
,5
]}

Zhang, Zhenyu ^{[3
,5
]}

Xie, Huiqi ^{[1
,2
,6
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Orthoped Surg, Lab Stem Cell & Tissue Engn,State Key Lab Biothera, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp, Orthoped Res Inst, State Key Lab Biotherapy,Lab Stem Cell & Tissue En, Chengdu 610041, Sichuan, Peoples R China

[3] Sichuan Univ, West China Hosp, Dept Plast & Burn Surg, Chengdu 610041, Sichuan, Peoples R China

[4] Capital Med Univ, Beijing Friendship Hosp, Med Cosmet Ctr, Beijing, Peoples R China

[5] Sichuan Univ, West China Tianfu Hosp, Dept Plast Reconstruct & Aesthet Surg, Chengdu 610041, Sichuan, Peoples R China

[6] Tianfu Jincheng Lab, Frontier Med Ctr, Chengdu, Sichuan, Peoples R China

来源：

CELL PROLIFERATION | 2024年 / 57卷 / 02期

基金：

中国国家自然科学基金;

关键词：

COLLAGEN TYPE-I; DORSAL AUGMENTATION; COSTAL CARTILAGE; CRUSHED CARTILAGE; OBLIQUE SPLIT; FASCIA LATA; RHINOPLASTY; DIFFERENTIATION; RECONSTRUCTION; PERICHONDRIUM;

D O I：

10.1111/cpr.13542

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cartilage absorption and calcification are prone to occur after the implantation of diced cartilage wrapped with autologous materials, as well as prolong the operation time, aggravate surgical trauma and postoperative pain during the acquisition process. Small intestinal submucosa (SIS) has suitable toughness and excellent degradability, which has been widely used in the clinic. Urine-derived stem cells (USCs), as a new type of stem cells, have multi-directional differentiation potential. In this study, we attempt to create the tissue engineering membrane material, termed USCs-SIS (U-SIS), and wrap the diced cartilage with it, assuming that they can promote the survival and regeneration of cartilage. In this study, after co-culture with the SIS and U-SIS, the proliferation, migration and chondrogenesis ability of the auricular-derived chondrocyte cells (ACs) were significantly improved. Further, the expression levels of chondrocyte phenotype-related genes were up-regulated, whilst that of dedifferentiated genes was down-regulated. The signal pathway proteins (Wnt3a and Wnt5a) were also participated in regulation of chondrogenesis. In vivo, compared with perichondrium, the diced cartilage wrapped with the SIS and U-SIS attained higher survival rate, less calcification and absorption in both short and long terms. Particularly, USCs promoted chondrogenesis and modulated local immune responses via paracrine pathways. In conclusion, SIS have the potential to be a new choice of membrane material for diced cartilage graft. U-SIS can enhance survival and regeneration of diced cartilage as a bioactive membrane material. Schematic illustration of the study design. In vitro, the effects of SIS and U-SIS on the behaviour of chondrocyte: cell proliferation, migration, chondrogenic ability, regulation of protein secretion and changes in genes and signal pathway protein related to cartilage phenotype. In vivo, perichondrium, SIS and U-SIS wrapped diced cartilage were used for grafting and their effect on cartilage repair was evaluated in a rabbit ear cartilage defect model.image

引用

页数：16