Mode of Action of Novel Pyrazoloquinazoline on Diamondback Moth (Plutella xylostella) Ligand-Gated Chloride Channels

被引：8
作者：
Li, Benjie [1 ]
Yan, Ying [2 ]
Yao, Guangkai [1 ]
Zhang, Ling [3 ,4 ]
Lin, Fei [1 ]
Xu, Hanhong [1 ]
机构：
[1] South China Agr Univ, Natl Key Lab Green Pesticide, Key Lab Nat Pesticide & Chem Biol, Minist Educ, Guangzhou 510642, Peoples R China
[2] Guangzhou Med Univ, Guangzhou Huiai Hosp, Affiliated Brain Hosp, Guangzhou, Peoples R China
[3] Jinan Univ, Inst Biomed, Coll Life Sci & Technol, Guangzhou 510632, Peoples R China
[4] Jinan Univ, Coll Life Sci & Technol, Dept Cell Biol, Guangzhou 510632, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 19期
基金：
中国国家自然科学基金;
关键词：
Plutella xylostella; pyrazoloquinazoline; mode of action; glutamate-gated chloride channel; gamma-aminobutyric acid receptor; DECREASES ABAMECTIN SUSCEPTIBILITY; RNA INTERFERENCE; FIPRONIL; RESISTANCE; LEPIDOPTERA; RECEPTOR; POPULATION; MECHANISMS; KNOCKDOWN; DYNAMICS;
D O I：
10.1021/acs.jafc.3c01270
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
In our previous study, a series of novel pyrazoloquinazolines were synthesized. Pyrazoloquinazoline 5a showed high insecticidal activity against the diamondback moth (Plutella xylostella) and no cross-resistance to fipronil. Patch clamp electrophysiology performed on P. xylostella pupae brains and two-electrode voltage clamp electrophysiology performed on Xenopus Laevis oocytes indicated that 5a might act on the ionotropic ?-aminobutyric acid (GABA) receptor (GABAR) and glutamate-gated chloride channel (GluCl). Moreover, 5a's potency on PxGluCl was about 15-fold higher than on fipronil, which may explain why there was no cross-resistance between 5a and fipronil. Downregulation of the PxGluCl transcription level significantly enhanced the insecticidal activity of 5a on P. xylostella. These findings shed light on the mode of action of 5a and provide important insights into the development of new insecticides for agricultural applications.
引用
收藏
页码：7250 / 7257
页数：8
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