Multifunctional Hydroxyapatite Nanobelt Haystacks Integrated Neural Stem Cell Spheroid for Rapid Spinal Cord Injury Repair

被引：10
作者：
Hao, Min [1 ]
Chen, Lu [2 ]
He, Jianlong [1 ]
Zhao, Xiaolei [1 ]
Xia, He [1 ]
Chen, Xin [1 ]
Yu, Liyang [1 ]
Qiu, Jichuan [1 ]
Feng, Shiqing [2 ]
Sang, Yuanhua [1 ]
Zhou, Hengxing [2 ]
Liu, Hong [1 ,3 ]
机构：
[1] Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China
[2] Shandong Univ, Qilu Hosp, Cheeloo Coll Med, Dept Orthopaed, Jinan 250012, Peoples R China
[3] Univ Jinan, Inst Adv Interdisciplinary Res IAIR, Jinan 250022, Peoples R China
来源：
ADVANCED FUNCTIONAL MATERIALS | 2023年 / 33卷 / 23期
基金：
中国国家自然科学基金;
关键词：
hybrid spheroids; hydroxyapatite nanobelts; neural stem cells; spinal cord injury repairs; MATRIX; DIFFERENTIATION; PROLIFERATION; SCAFFOLD; P53;
D O I：
10.1002/adfm.202214869
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Due to the unwarranted lifespan and differentiation, applying neural stem cells (NSCs) in spinal cord injury (SCI) remains challenging. In this study, 3D bioactive hydroxyapatite (HAp) nanobelt haystack-mouse NSC (mNSC) hybrid spheroids are customized in which the specific nanobelt haystack framework provided the structural function of hypoxia alleviation in the spherical core and biological process of neural differentiation promotion. Commodified with superparamagnetic ferroferric oxide (Fe3O4) nanoparticles and a polydopamine (PDA) coating, the HAp nanobelts are endowed with magnetic field-driven properties and enhanced cell-nanobelt adhesion. The engineered bioresponsive 3D nanobelt haystack-mNSC hybrid spheroids effectively repair SCI in vivo, showing new potential for stem cell therapy by incorporating nanomaterials in 3D culture based on cell-material interactions.
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页数：14
相关论文
共 51 条
  • [31] Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries
    Rathnam, Christopher
    Yang, Letao
    Castro-Pedrido, Sofia
    Luo, Jeffrey
    Cai, Li
    Lee, Ki-Bum
    [J]. SCIENCE ADVANCES, 2021, 7 (40)
  • [32] Advancing models of neural development with biomaterials
    Roth, Julien G.
    Huang, Michelle S.
    Li, Thomas L.
    Feig, Vivian R.
    Jiang, Yuanwen
    Cui, Bianxiao
    Greely, Henry T.
    Bao, Zhenan
    Pasca, Sergiu P.
    Heilshorn, Sarah C.
    [J]. NATURE REVIEWS NEUROSCIENCE, 2021, 22 (10) : 593 - 615
  • [33] Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells
    Ryu, Na-Eun
    Lee, Soo-Hong
    Park, Hansoo
    [J]. CELLS, 2019, 8 (12)
  • [34] A chemical method for fast and sensitive detection of DNA synthesis in vivo
    Salic, Adrian
    Mitchison, Timothy J.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (07) : 2415 - 2420
  • [35] Opportunities and challenges of pluripotent stem cell neurodegenerative disease models
    Sandoe, Jackson
    Eggan, Kevin
    [J]. NATURE NEUROSCIENCE, 2013, 16 (07) : 780 - 789
  • [36] Biomaterial Cues Regulated Differentiation of Neural Stem Cells into GABAergic Neurons through Ca2+/c-Jun/TLX3 Signaling Promoted by Hydroxyapatite Nanorods
    Shen, Yinan
    Liu, Feng
    Duan, Jiazhi
    Wang, Wenhan
    Yang, Hongru
    Wang, Zizhao
    Wang, Tailin
    Kong, Ying
    Ma, Baojin
    Hao, Min
    Zhao, Hang
    Liu, Hong
    [J]. NANO LETTERS, 2021, 21 (17) : 7371 - 7378
  • [37] Tamrin S. H., 2017, ADV FUNCT MATER, V27
  • [38] Mechanical forces direct stem cell behaviour in development and regeneration
    Vining, Kyle H.
    Mooney, David J.
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2017, 18 (12) : 728 - 742
  • [39] Wang LH, 2013, NAT METHODS, V10, P84, DOI [10.1038/NMETH.2283, 10.1038/nmeth.2283]
  • [40] A bioinspired scaffold for rapid oxygenation of cell encapsulation systems
    Wang, Long-Hai
    Ernst, Alexander Ulrich
    An, Duo
    Datta, Ashim Kumar
    Epel, Boris
    Kotecha, Mrignayani
    Ma, Minglin
    [J]. NATURE COMMUNICATIONS, 2021, 12 (01)
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