Genome-Enabled Analysis of Population Dynamics and Virulence-Associated Loci in the Oat Crown Rust Fungus Puccinia coronata f. sp. avenae

被引：7

作者：

Hewitt, Tim C. ^{[1
]}

Henningsen, Eva C. ^{[1
]}

Pereira, Danilo ^{[2
,3
]}

McElroy, Kerensa ^{[1
]}

Nazareno, Eric S. ^{[4
]}

Dugyala, Sheshanka ^{[4
]}

Nguyen-Phuc, Hoa ^{[4
,9
]}

Li, Feng ^{[4
,7
]}

Miller, Marisa E. ^{[4
,8
]}

Visser, Botma ^{[5
]}

Pretorius, Zacharias A. ^{[5
]}

Boshoff, Willem H. P. ^{[5
]}

Sperschneider, Jana ^{[1
]}

Stukenbrock, Eva H. ^{[2
,3
]}

Kianian, Shahryar F. ^{[4
,6
]}

Dodds, Peter N. ^{[1
]}

Figueroa, Melania ^{[1
]}

机构：

[1] Commonwealth Sci & Ind Res Org, Agr & Food, Canberra, ACT 2600, Australia

[2] Christian Albrechts Univ Kiel, D-24118 Kiel, Germany

[3] Max Planck Inst Evolutionary Biol, D-24306 Plon, Germany

[4] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA

[5] Univ Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa

[6] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA

[7] eGenesis, 101 Cambridge Pk Dr, Cambridge, MA 02140 USA

[8] Pairwise Plants LLC, Durham, NC USA

[9] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2024年 / 37卷 / 03期

基金：

美国食品与农业研究所;

关键词：

disease; effector; genetic variation; oat; rust; virulence; MOLECULAR MARKERS; RESISTANCE; WHEAT; INHERITANCE; AVIRULENCE; PROTEIN; GENES; IDENTIFICATION; ACCESSIONS; PATHOGEN;

D O I：

10.1094/MPMI-09-23-0126-FI

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Puccinia coronata f. sp. avenae (Pca) is an important fungal pathogen causing crown rust that impacts oat production worldwide. Genetic resistance for crop protection against Pca is often overcome by the rapid virulence evolution of the pathogen. This study investigated the factors shaping adaptive evolution of Pca using pathogen populations from distinct geographic regions within the United States and South Africa. Phenotypic and genome-wide sequencing data of these diverse Pca collections, including 217 isolates, uncovered phylogenetic relationships and established distinct genetic composition between populations from northern and southern regions from the United States and South Africa. The population dynamics of Pca involve a bidirectional movement of inoculum between northern and southern regions of the United States and contributions from clonality and sexuality. The population from South Africa is solely clonal. A genome-wide association study (GWAS) employing a haplotype-resolved Pca reference genome was used to define 11 virulence-associated loci corresponding to 25 oat differential lines. These regions were screened to determine candidate Avr effector genes. Overall, the GWAS results allowed us to identify the underlying genetic factors controlling pathogen recognition in an oat differential set used in the United States to assign pathogen races (pathotypes). Key GWAS findings support complex genetic interactions in several oat lines, suggesting allelism among resistance genes or redundancy of genes included in the differential set, multiple resistance genes recognizing genetically linked Avr effector genes, or potentially epistatic relationships. A careful evaluation of the composition of the oat differential set accompanied by the development or implementation of molecular markers is recommended. Copyright (c) 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

引用

页码：290 / 303

页数：14

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