Kaempferitrin alleviates LPS-induced septic acute lung injury in mice through downregulating NF-κB pathway

被引:2
作者
Zhu, Xiaoli [1 ]
Pan, Yongyue [1 ]
Xu, Xin [2 ]
Xu, Jing [1 ]
机构
[1] Zhejiang Youth Hosp, Crit Care Med, 54 Qingchun East Rd,Kaixuan St, Hangzhou 310000, Zhejiang, Peoples R China
[2] Zhejiang Chinese Med Univ, Crit Care Med, Hangzhou, Zhejiang, Peoples R China
关键词
acute lung injury; kaempferitrin; lipopolysaccharide; NF-kappa B pathway; sepsis;
D O I
10.15586/aei.v51i6.838
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Acute lung injury (ALI) causes severe and uncontrolled pulmonary inflammation and has high morbidity in dying patients. Objective: This study aimed to evaluate the potential function of Kaempferitrin (Kae) and uncover its mechanisms in ALI. Material and Methods: We evaluated the role of Kae in ALI through the lipopolysaccharide lavage fluid (BALF) cells count, pulmonary inflammation, and the levels of interleukin (IL)-6, tumor necrosis factor-alpha (TNF-alpha), and IL-1 beta. The effect of Kae on NF-kappa B signaling pathway was discovered through the protein expression levels of transcription factors p65, p-p65, I kappa B alpha, and p-I kappa B alpha by Western blot analysis. Results: The results showed that Kae could improve lung injury by reducing apoptosis, histopathological changes, and lung W/D ratio; more importantly, Kae enhanced the survival of ALI mice. Moreover, Kae relieved inflammation, as it reduced total BALF cells count, and deceased the levels of TNF-alpha, IL-6, and IL-1 beta in serum. In addition, Western blot analysis data suggested that Kae could decrease the protein expression levels of transcription factors p65, p-p65, I kappa B-alpha, and p-I kappa B-alpha, which were promoted by LPS. Conclusion: The results of this study suggested that Kae could relieve LPS-induced ALI in mice and reduce inflammation and apoptosis through NF-kappa B pathway. (c) 2023 Codon Publications. Published by Codon Publications.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 50 条
  • [41] Amygdalin alleviates LPS-induced acute lung injury in mice by targeting CD5L/iNOS pathway
    Zhou, Bo
    Xue, Jiahui
    Wang, Jing
    Yu, Donghua
    Zhou, Fangling
    Duan, Jin-ao
    Niu, Yang
    Wang, Hanqing
    MOLECULAR IMMUNOLOGY, 2024, 176 : 22 - 29
  • [42] Deoxyelephantopin alleviates lipopolysaccharide-induced septic lung injury through inhibiting NF-κB/STAT3 axis
    Wang, Shu
    Chen, Yuefeng
    ALLERGOLOGIA ET IMMUNOPATHOLOGIA, 2022, 50 (05) : 39 - 46
  • [43] Allyl methyl trisulfide protected against LPS-induced acute lung injury in mice via inhibition of the NF-κB and MAPK pathways
    Wang, Shuo
    Liu, Jinqian
    Dong, Jing
    Fan, Zongqiang
    Wang, Fugui
    Wu, Ping
    Li, Xiaojing
    Kou, Ruirui
    Chen, Fang
    FRONTIERS IN PHARMACOLOGY, 2022, 13
  • [44] Shensongyangxin protects cardiomyocytes against LPS-induced injury through the NFκB pathway
    Shen, Di-Fei
    Ni, Jian
    Wu, Qing-Qing
    Deng, Wei
    Wei, Cong
    Jia, Zhen-Hua
    Zhou, Heng
    Zhou, Meng-Qiao
    Bian, Zhou-Yan
    Tang, Qi-Zhu
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2016, 9 (02): : 2317 - 2324
  • [45] Continuous Activation of Dopamine Receptors Alleviates LPS-Induced Liver Injury in Mice via β-arrestin2 Dependent Akt/NF-κB Pathway
    Li, Mingan
    Zhang, Ce
    Zhou, Lin
    Sun, Xiaohui
    Wang, Tian
    Fu, Fenghua
    FRONTIERS IN PHARMACOLOGY, 2022, 13
  • [46] Peimine ameliorates LPS-induced acute lung injury by regulating Nrf2 and NF-κB pathways
    Huang, Hui
    Wang, Guanhua
    Zeng, Dali
    AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2024, 16 (10): : 5385 - 5397
  • [47] Mogroside IIIE Attenuates LPS-Induced Acute Lung Injury in Mice Partly Through Regulation of the TLR4/MAPK/NF-κB Axis via AMPK Activation
    Tao, Lijun
    Cao, Fengyan
    Xu, Gonghao
    Xie, Haifeng
    Zhang, Mian
    Zhang, Chaofeng
    PHYTOTHERAPY RESEARCH, 2017, 31 (07) : 1097 - 1106
  • [48] Geraniol alleviates LPS-induced acute lung injury in mice via inhibiting inflammation and apoptosis
    Jiang, Kangfeng
    Zhang, Tao
    Yin, Nannan
    Ma, Xiaofei
    Zhao, Gan
    Wu, Haichong
    Qiu, Changwei
    Deng, Ganzhen
    ONCOTARGET, 2017, 8 (41) : 71038 - 71053
  • [49] Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway
    Wu, Xialei
    Lin, Liyao
    Wu, Haibin
    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2021, 35 (03)
  • [50] Class B Scavenger Receptors BI and BII Protect against LPS-Induced Acute Lung Injury in Mice by Mediating LPS
    Baranova, Irina N.
    Bocharov, Alexander, V
    Vishnyakova, Tatyana G.
    Chen, Zhigang
    Birukova, Anna A.
    Ke, Yunbo
    Hu, Xuzhen
    Yuen, Peter S. T.
    Star, Robert A.
    Birukov, Konstantin G.
    Patterson, Amy P.
    Eggerman, Thomas L.
    INFECTION AND IMMUNITY, 2021, 89 (10)